基质金属蛋白酶-3血清水平及启动子基因5A／6A多态性与动脉粥样硬化性脑梗死的关系

Association of matrix metalloproteinase-3 sermn level and the promoter 5A/6A polymorphism of the MMP-3 gene with atherosclerotic cerebral infarction

目的探讨基质金属蛋白酶．3（matrix metalloproteinase-3，MMP-3）血清水平及启动子基因5A／6A多态性与动脉粥样硬化性脑梗死（atherosclerotic cerebral infarction，ACI）的关系。方法对215例ACI患者和226名健康体检者进行研究。采用ELISA法测定血清MMP-3水平，同时采用聚合酶链反应-限制性片段长度多态性方法分析MMP-3启动子基因5A／6A多态性。结果ACI组发病48h内的血清MMP-3水平为（23．28±12．19）μg／μL，较对照组（2．76±2．25）ng／μL显著增高（t=24．867，P=0．000）。ACI组内5A／6A＋5A／5A基因型患者外周血MMP-3水平为（26．04±11．79）μg／μL，6A／6A基因型患者外周血MMP-3水平为（22．24±12．21）ng／mL，两者间差异有统计学意义（t=2．057，P=0．041）。ACI组5A／6A＋5AV5A基因型频率为27．4％，对照组为15．5％，两者比较差异有统计学意义（x2=9．389，P=0．002），5A等位基因频率在ACI组为14．2％，对照组为7．7％，两者比较差异也有统计学意义（x2=9．430，P=0．002）。结论ACI患者发病48h内血清MMP-3水平显著增高，MMP-3启动子基因5A／6A多态性可能与MMP3的血清水平及ACI发生倾向性有关。

Objective To investigate the association of matrix metalloproteinase-3 （MMP-3） serum level and the promoter 5A/6A polymorphism of the MMP-3 gene with atherosclerotic cerebral infarction （ACI） in a Chinese Han population. Methods Two hundred and fifteen patients with acute ACI from the Department of Neurology of Taizhou Hospital and 226 healthy controls were included in the study. Serum MMP-3 level was measured by enzyme-linked immunosorbent assay （ELISA）. Genotype was determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） for the common 5A/6A functional promoter polymorphism of the MMP-3 gene. Results The genotype distribution of the MMP-3 promoter 5A/6A polymorphism between the ACI patients group and the control group was sig- nificantly different（x2 = 9. 389, P = O. 002 ）. The frequencies of the 5A allele were 14.2 % and 7.7 % in the ACI patients group and the control group respectively（x2 = 9.430, P = 0.002）. Serum level of MMP-3 in the ACI patients group was significantly higher than that in the control group（ t = 24. 867, P = 0. 000）. Among the ACI patients group, serum MMP- 3 levels also had significant difference between the 5A/6A ＋ 5A/5A and the 6A/6A genotype（ t = 2.057, P = O. 041 ）. Conclusion The present findings suggest that serum level of MMP-3 obviously increased within 48 hours of ischemic stroke and the genetic polymorphism of 5A/6A in the MMP-3 promoter is associated with ACI and MMP-3 expression in the Chinese Han population.