乙型肝炎病毒感染不同时期患者外周血树突状细胞亚群数量的变化

Quantitive changes of circulating myeloid and plasmacytoid dendritic cell subsets in HBV infected patients during different periods of their infection

目的探讨HBV感染患者不同感染时期（免疫耐受期、免疫活动期、非复制期）的树突状细胞（DC）亚群髓样树突状细胞（mDC）和浆细胞样树突状细胞（pDC）数量的差异及其与外周血HBVDNA水平的相关性。方法收集HBV感染不同阶段的患者30例，分为免疫耐受组、免疫活动组、非复制组各10例，另选健康人10名作为对照组。取4组患者的抗凝血10ml，提取外周血单个核细胞，然后用流式细胞仪检测并计算出患者外周血DC亚群的频率（百分比）和数量。统计学分析不同组间DC亚群频率、数量变化及其与HBVDNA的相关性。结果HBV感染组mDC绝对值、pDC百分比、pDC绝对值分别为（9．40±4．16）×10^6／L、0．17％±0．10％、（3．77±2．44）×10^6／L，均低于健康对照组，差异有统计学意义（P〈0．05）。健康对照组mDC百分比（0．56％±0．11％）高于免疫耐受组（0．47％±0．19％），mDC绝对值[（14．81±4．75）×10^6／L]高于免疫耐受组[（9．50±3．98）×10^6／L]和免疫活动组[（8．44±2．66）×10^6／L]，pDC百分比（0．26％±0．08％）高于免疫活动组（0．14％±0．10％）和非复制组（0．18％±0．11％），pDC绝对值[（16．65±2．01）×10^6／L]高于其他3组，差异均有统计学意义（P〈0．05）。HBV感染不同组别中仅免疫耐受组pDC细胞数与HBVDNA水平呈负相关关系（P〈0．05）。结论HBV感染不同时期mDC和pDC频率和数量较健康人群下降。不同组DC亚群数量中仅免疫耐受组pDC细胞数与HBVDNA水平呈负相关。

Objective To study the quantities ofmyeloid and plasmacytoid dendritic cell （mDC, pDC） subsets associated with different blood virus loads （HBV DNA） in patients in different periods of HBV infection （immune tolerance, immune activities and non-replicating periods）. Methods Thirty HBV infected patients in different stages of infection were enrolled. They were divided into three groups： an immune tolerance group （10 cases）, an immunization activities group （10 cases）, and a non-replication group （10 cases）. Ten healthy people were enrolled and served as controls. Blood （10ml） from the patients and the controls were collected and the numbers of dentritic cells （DC） in percentage were counted using flow cytometry. The numbers ofmDC and pDC were counted and the relevance of them with their blood virus loads （HBV DNA） was analyzed using statistical methods. Results （1） Absolute values of mDC, pDC percentage and pDC absolute value of the HBV infected patients were lower than those of the healthy control group （P 〈 0.05）. mDC percentage of the HBV infected patients was a little lower than that of the healthy controls but of no statistical significance （P 〉 0.05）. （2） The percentage ofmDC in the healthy controls was higher than that in the tolerance group （P 〈 0.05） and its absolute value in the healthy control group was higher than those in the immune tolerance group and the immune activities group （P 〈 0.05）. pDC percentage of the healthy control group was higher than those of the immune activities and non-reproduction groups （P 〈 0.05）. pDC absolute value of the healthy control group was higher than those of the immune tolerance, immune activities and non- reproduction groups （P 〈 0.05）. （3） The pDC counts in the tolerance group were negatively correlated with HBV DNA levels （r = -0.686, P 〈 0.05）. Conclusions （1） DC fi＇equency and counts of HBV infected patients in different periods decreased. The immune active group decreased significantly more than the healthy control group （P 〈 0.05）. （2） pDC counts show a negative correlation with HBV DNA levels in only the tolerance group.