摘要
利用Dular和Lemont杂交后代单粒传衍生的123个F12家系所组成的重组自交系(RILs)群体,研究水稻剑叶叶绿素含量的数量性状基因座(QTL).分别在2005年和2006年考察该RIL群体齐穗期剑叶叶绿素含量,并进行QTL定位和上位性分析及其与环境的互作效应分析.结果表明:在4对染色体上共检测到10个控制叶绿素含量的加性QTLs,共解释了73.51的遗传变异,单个QTL的表型贡献率为2.08~20.14,其中6个和环境存在显著互作;同时也检测到13对影响叶绿素含量的加性×加性上位性互作,其中6对具有显著的上位性环境互作效应.
By using a population of 123 F12 lines (recombinant inbred lines, RILs) derived from a cross between India variety Dular and Japanica variety Lemont, an analysis of quantitative trait loci (QTL) was conducted for the flag leaf chlorophyll content of rice. The chlorophyll content was determined by SPAD-502 in 2005 and 2006, respectively, and software QTLMapper 1.6 was applied to analyze the QTL, including the additive and epistatic effects and the QTL interactions with environment for chlorophyll content. A total of ten QTL showing additive effects on chlorophyll content (Chl) were detected, which accounted for 73.51% of the phenotypic variation. The percentage of phenotypic variation explained by single QTL was 2. 08%--20. 14% , and the interactions of 6 QTLs with environment (AE) were significant. Epistasis analysis indicated that there existed 13 significant additive × additive interactions on chlorophyll content, 6 pairs of which were significant in additive × additive interactions with environment (AAE).
出处
《应用生态学报》
CAS
CSCD
北大核心
2008年第12期2651-2655,共5页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(30571103
39970441
30471028
30070068)
教育部生态学博士点基金资助项目(20050389004)