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survivin在皮肤血管瘤组织中的表达及其与半胱氨酸天冬氨酸蛋白酶-3的关系

Expression of survivin in human dermal hemangioma and its relationship with caspase-3
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摘要 目的检测凋亡抑制因子survivin在各期皮肤真性血管瘤中的表达及其与半胱氨酸天冬氨酸蛋白酶-3（caspase-3）表达的关系，探讨其在血管瘤发病机制中的作用以及在临床分子治疗中的意义。方法利用免疫组织化学SP染色法显示survivin、caspase-3在增生期、退化期血管瘤组织和正常皮肤组织中的表达，并运用病理图文报告管理系统处理染色结果，比较各组表达有无差异，分析两指标间的关系。结果survivin在增生期血管瘤、退化期血管瘤和正常皮肤组织中的吸光度值分别是0．2449±0．0135、0．1648±0．0217和0．1789±0．0126，增生期组与其他两组间差异有统计学意义（P〈0．01）；而caspase-3在退化期血管瘤中的表达明显高于增生期血管瘤组织和正常皮肤（P〈0．01）。在增生期和退化期血管瘤组织中，survivin与caspase-3的表达呈统计学负相关性（r=-0．95、P〈0．01）。结论survivin通过在增生期血管瘤中高表达，抑制caspase-3的激活来调控血管内皮细胞的凋亡，在皮肤血管瘤的病理演变过程中发挥着重要作用。 Objective To investigate the expression of survivin and its relationship with that of caspase-3 in two stages of hemangioma and normal skin, and to explore the role of survivin in the pathogenesis of hemangioma. Methods A total of 50 cases of human dermal hemangioma and 8 cases of normal skin were analyzed for expression of survivin and caspase-3 by immunohistochemistry. Results The positive ratio and average light density of survivin were obviously higher in proliferative hemangioma （0.2449±0.0135,0. 7246±0. 0747） than that in involutional hemangioma （0. 1648±0. 0217, 0.5592±0.1601） and normal skin（0.1789±0.0126,0.4626±0.0961） （P〈0.01）. On the contrary, the expression of caspase-3 was up-regulated in involutional hemangioma （0. 2386 ±0. 0175, 0. 4378±0. 0593）. Analysis of data also showed that there was a negative correlation between the average light density of survivin and caspase 3 （r= -0.95 ,P〈0.01）. Conclusions Survivin plays an important role in the pathogenesis of hemangioma and has a negative relationship with caspase-3.
作者 刘思洋 叶玲 陕声国 张端莲
机构地区 武汉大学人民医院整形外科 武汉大学医学院组织胚胎学教研室
出处 《中华医学美学美容杂志》 2008年第5期326-329,共4页 Chinese Journal of Medical Aesthetics and Cosmetology
基金 国家自然科学基金资助项目（编号：30271345）
关键词 SURVIVIN 半胱氨酸天冬氨酸蛋白酶-3 血管瘤 免疫组织化学 Survivin Caspase-3 Hemangioma Immunohistochemistry
分类号 R73 [医药卫生—肿瘤]
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参考文献11

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二级参考文献16

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中华医学美学美容杂志
2008年 第5期

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