摘要
AIM:To investigate the effect of hepatoma cells on up-regulation of programmed cell death-1 (PD-1),and the function of PD-1 on T cells. METHODS:HepG2 or HepG2.2.1.5 cells were co-cultured with a lymphoma cell line-Jurkat cells. PD-1 expression was detected by flow cytometry. IL-2,INF-γ and IL-10 in culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA). Cytotoxic action of T cells was determined by MTT reduction assay-direct mononuclear cell cytotoxicity assay. RESULTS:The PD-1 expression on Jurkat cells increased by 16.17% ± 2.5% and 17.43% ± 2.2% after HepG2 or HepG2.2.1.5 cells were co-cultured for 48 h. The levels of IL-2,INF-γ and IL-10 in the culture supernatant were 202.9 ± 53.0 pg/mL,88.6 ± 4.6 pg/mL and 63.7 ± 13.4 pg/mL respectively,which were signif icantly higher than those (102.9 ± 53 pg/mL,39.3 ± 4.2 pg/mL,and 34.6 ±13.7 pg/mL) in the control group (P < 0.05). The OD value for MTT assay in the blocking group (0.29 ± 0.06) was significantly higher than that (0.19 ± 0.09) in the control group (P < 0.05). CONCLUSION:PD-1 expression on Jurkat cells is up-regulated by hepatoma cells,cytokines and cytotoxic action are elevated after PD-1/PD-L1 is blocked.
AIM: To investigate the effect of hepatoma cells on up-regulation of programmed cell death-1 (PD-1), and the function of PD-1 on T cells. METHODS: HepG2 or HepG2.2.1.5 cells were cocultured with a lymphoma cell line-Jurkat cells. PD-1 expression was detected by flow cytometry. IL:2, INF-γ and IL-10 in culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA). Cytotoxic action of T cells was determined by MIF reduction assay-direct mononuclear cell cytotoxicity assay. RESULTS: The PD-1 expression on Jurkat cells increased by 16.17% ± 2.5% and 17.43% ± 2.2% after HepG2 or HepG2.2.1.5 cells were co-cultured for 48 h. The levels of IL-2, INF-γ and IL-10 in the culture supernatant were 202.9 + 53.0 pg/mL, 88.6 ± 4.6 pg/mL and 63.7± 13.4 pg/mL respectively, which were significantly higher than those (102.9 ± 53 pg/mL, 39.3 ± 4.2 pg/mL, and 34.6 =E13.7 pg/mL) in the control group (P 〈 0.05). The OD value for MTT assay in the blocking group (0.29 ± 0.06) was significantly higher than that (0.19 ± 0.09) in the control group (P 〈 0.05). CONCLUSION: PD-1 expression on Jurkat cells is upregulated by hepatoma cells, cytokines and cytotoxic action are elevated after PD-1/PD-L1 is blocked.
基金
Supported by National Natural Science Foundation of China, No. 30771905
关键词
肝细胞瘤
细胞死亡
蛋白表达
T细胞功能
Hepatoma cell
Programmed cell death-l
Protein expression
T cell function
Cytokine