摘要
AIM:To explore the effects of recombinant human growth hormone (rhGH) on intestinal mucosal epithelial cell proliferation and nutritional status in patients with enterocutaneous fi stula. METHODS:Eight patients with enterocutaneous fi stulas received recombinant human growth hormone (10 μg/d) for 7 d. Image analysis and immunohisto-chemical techniques were used to analyse the expression of proliferating cell nuclear antigen (PCNA) in intestinal mucosal epithelial cells in biopsy samples from the patients who had undergone an endoscopic biopsy through the fi stula at day 0,4 and 7. Body weights,nitrogen excretion,serum levels of total proteins,albumin,prealbumin,transferrin and fi bronectin were measured at day 0,4 and 7. RESULTS:Significant improvements occurred in the expression of PCNA in the intestinal mucosal epithelial cells at day 4 and 7 compared to day 0 (24.93 ± 3.41%,30.46 ± 5.24% vs 12.92 ± 4.20%,P < 0.01). These changes were accompanied by the significant improvement of villus height (500.54 ± 53.79 μm,459.03 ± 88.98 μm vs 210.94 ± 49.16 μm,P < 0.01),serum levels of total proteins (70.52 ± 5.13 g/L,74.89 ± 5.16 g/L vs 63.51 ± 2.47 g/L,P < 0.01),albumin (39.44 ± 1.18 g/L,42.39 ± 1.68 g/L vs 35.74 ± 1.75 g/L,P < 0.01) and f ibronectin (236.3 ± 16.5 mg/L,275.8 ± 16.9 mg/L vs 172.5 ± 21.4 mg/L,P < 0.01) at day 4 and 7,and prealbumin (286.38 ± 65.61 mg/L vs 180.88 ± 48.28 mg/L,P < 0.05),transferrin (2.61 ± 0.12 g/L vs 2.41 ± 0.14 g/L,P < 0.05) at day 7. Nitrogen excretion was signifi cantly decreased at day 7 (3.40 ± 1.65 g/d vs 7.25 ± 3.92 g/d,P < 0.05). No change was observed in the body weight. CONCLUSION:Recombinant human growth hormone could promote intestinal mucosal epithelial cell proliferation and protein synthesis in patients with enterocutaneous fi stula.
AIM: To explore the effects of recombinant human growth hormone (rhGH) on intestinal mucosal epithelial cell proliferation and nutritional status in patients with enterocutaneous fistula. METHODS: Eight patients with enterocutaneous fistulas received recombinant human growth hormone (10 ug/d) for 7 d. Image analysis and immunohistochemical techniques were used to analyse the expression of proliferating cell nuclear antigen (PCNA) in intestinal mucosal epithelial cells in biopsy samples from the patients who had undergone an endoscopic biopsy through the fistula at day 0, 4 and 7. Body weights, nitrogen excretion, serum levels of total proteins, albumin, prealbumin, transferrin and fibronectin were measured at day 0, 4 and 7. RESULTS: Significant improvements occurred in the expression of PCNA in the intestinal mucosal epithelial cells at day 4 and 7 compared to day 0 (24.93 ± 3.41%, 30.46 ± 5.24% vs 12.92 ± 4.20%, p 〈 0.01). These changes were accompanied by the significant improvement of villus height (500.54 ± 53.79 um, 459.03 ± 88.98um vs 210.94 ± 49.16 um, P 〈 0.01), serum levels of total proteins (70.52 ± 5.13 g/L, 74.89 ± 5.16 g/L vs 63.51 ± 2.47 g/L, P 〈 0.01), albumin (39.44 ± 1.18 g/L, 42.39 ± 1.68 g/L vs 35.74 ± 1.75 g/L, P 〈 0.01) and fibronectin (236.3 4- 16.5 mg/L, 275.8± 16.9 mg/L vs 172.5 ± 21.4 mg/L, P 〈 0.01) at day 4 and 7, and prealbumin (286.38 ± 65.61 mg/L vs 180.88 ± 48.28 mg/L, P 〈 0.05), transferrin (2.61 ± 0.12 g/L vs 2.41 ±0.14 g/L, P 〈 0.05) at day 7. Nitrogen excretion was significantly decreased at day 7 (3.40 ± 1.65 g/d vs 7.25 ± 3.92 g/d, P 〈 0.05). No change was observed in the body weight. CONCLUSION: Recombinant human growth hormone could promote intestinal mucosal epithelial cell proliferation and protein synthesis in patients with enterocutaneous fistula.
基金
Supported by National Natural Science Foundation of China, No. 30571797
National Natural Science Foundation of Jiangsu Province, No. BK2006719
关键词
人类
生长激素
重组体
上皮细胞
细胞核心抗原
Recombinant human growth hormone
Enterocutaneous fistula
Intestinal
Epithelial cell
Proliferating cell nuclear antigen