乌蕨孢子萌发研究

Investigation on Spore Germination of Sphenomeris chinensis(Lindsaeaceae)

用随机区组试验和方差分析方法,研究了培养温度、贮藏温度、GA处理和光照强度对乌蕨(Sphe-nomeris chinensis)孢子萌发的影响。与室温(20～25℃)相比,培养温度在28℃时,孢子最大萌发率相近而萌发速率明显较高。贮藏温度(A)极显著(P<0.01)影响孢子萌发率,-20℃贮藏降低萌发率;GA(B)对孢子萌发率无显著影响。光照强度(C)极显著(P<0.01)影响孢子萌发率,充足光照和弱光照无显著差异,黑暗处理降低萌发率。A×B,A×C,B×C及A×B×C交互效应不显著。

Effects of cuhure temperature, storage temperature, GA treatment and illumination intensity on spore germination of Sphenomeris chinensis were studied using randomized blocks experiments and analysis of variance. Compared with room temperature （20-25℃）, the germination velocity was higher at the culture temperature of 28℃, although their maximal germination percentage was the same. Storage temperature （A） significantly （ P 〈 0.01） affected spore germination, and germination of spores was reduced when stored at -20℃. GA （B） did not affect germination. Illumination intensity （C） significantly （ P 〈 0.01 ） affected germination. There were no significant difference between full and weak illumination, but the dark reduced germination percentage. A × B, A × C, B × C and A × B × C had no significant interaction effect.