基质金属蛋白酶及其组织抑制剂在牙本质生成性影细胞瘤和牙源性影细胞癌中的表达

Roles of matrix metalloproteinases and tissue specific inhibitors of metalloproteinases in dentinogenic ghost cell tumor and ghost cell odontogenic carcinoma

目的对牙本质生成性影细胞瘤（dentinogenic ghost cell tumor，DGCT）和牙源性影细胞癌（ghost cell odontogenic carcinoma，GCOC）中基质金属蛋白酶（matrix metalloproteinases，MMP）和金属蛋白酶组织抑制剂（tissue inhibitors of metalloproteinase，TIMP）的表达特点进行研究。方法免疫组织化学分析15例DGCT和9例GCOC中MMP-2、MMP-9、MMP-14、TIMP-1和TIMP-2的表达。对DGCT和GCOC各一例分析MMP-2、MMP-9、MMP-14、TIMP-1和TIMP-2mRNA的表达；明胶酶谱分析MMP一2和MMP-9酶原和活化蛋白的表达。结果MMPO和TIMP-1在GCOC中高表达（7／9，8／9）；TIMP-1在GCOC中的高表达，与在DGCT中相比差异具有统计学意义（P〈0．05）。对一例DGCT和一例GCOC病例研究发现，MMPO酶原和活酶在GCOC中高表达，并且MMP-9和TIMP-1mRNA在GCOC中高表达。结论MMPO和TIMP-1可能与GCOC的性质和生物学行为有关。

Objective To investigate the roles of matrix metalloproteinases （MMP） and tissue inhibitors of metalloproteinases （TIMP） in dentinogenic ghost cell tumor（DGCT） and ghost cell odontogenic carcinoma（GCOC）. Methods The expressions of MMP-2, MMP-9, MMP-14, TIMP-1 and TIMP-2 were examined in 15 DGCT cases and 9 GCOC cases by immunohistochemistry. Their mRNA expression in one DGCT case and one GCOC ease were investigated by RT-PCT. MMP-2 and MMP-9 protein activities in the two cases were analyzed by gelatin zymography. Results MMP-9 and TIMP-1 expressions elevated greatly in GCOC, and there was a significant difference （P 〈 0.05 ） in TIMP-1 expression between GCOC and DGCT. Pro-MMP-9, MMP-9 activated form, pro-MMP-2, and MMP-2 activated forms were detected in the GCOC case, while pro-MMP-9 and MMP-9 activated form were very faint in the DGCT case. The mRNA level of MMP-9 elevated obviously in the GCOC case, which was similar to that of TIMP-1. Conclusions The elevated expression of MMP-9 and TIMP-1 may influence the behaviour of GCOC.