摘要
为研究和获得红菜薹线粒体DNA中与细胞质雄性不育相关的片段,对其不育系和保持系的线粒体DNA进行了RAPD分析。结果表明,在285条引物的扩增图谱中,不育系和保持系的线粒体DNA之间存在差异,在不育系中获得两条特异带AY1200和RD800;序列分析发现,AY1200与拟南芥的铁氧化还原蛋白依赖的谷氨酸盐合成酶的65个氨基酸有33%的相似性;RD800序列与贝氏柯克斯体QpH1质粒全基因组序列的132个氨基酸有47%的相似性。
In order to study and obtain special fragments related to CMS in mitochondria DNA of the Pol CMS of Purple Flowering Stalk, the mitochondrial DNA of the Pol CMS and its maintainer line was analyzed by RAPD. With 285 RAPD random primers two specific fragments were obtained in the CMS line. Sequence analysis showed that RDS00 was of 47 % of homology with 132 amino acids of Coxiella bnrnetii QpH1 plasmid genome, AY1200 was of 33 % of homology with 65 amino acids of ferredoxase-dependent GLU2 of Arabidopsis thalianca.
出处
《湖北农业科学》
北大核心
2008年第11期1232-1234,共3页
Hubei Agricultural Sciences
基金
湖北省农业科技创新中心资助项目(2007-620-001-03)
