MMP-1和TIMP-1在人胃癌组织中的表达及临床意义

Expression and significance of MMP-1 and TIMP-1 genes in gastric cancer

目的探讨基质金属蛋白酶1(MMP-1)和金属蛋白酶1组织抑制因子(TI MP-1)基因与胃癌临床生物学行为的关系。方法采用逆转录-聚合酶链反应(RT-PCR)检测60例胃癌组织及其相应癌旁正常胃黏膜组织中MMP-1和TI MP-1 mRNA的表达。结果60例胃癌组织中MMP-1表达水平明显高于癌旁正常组织,TI MP-1表达水平明显低于癌旁正常组织(P<0.05)。胃癌组织中,MMP-1 mRNA的相对表达强度、未分化癌明显高于高、中分化癌,Ⅲ～Ⅳ期明显高于Ⅰ～Ⅱ期,伴淋巴结转移者明显高于无淋巴结转移者(P<0.05);TI MP-1 mRNA的相对表达强度低、未分化癌则明显低于高、中分化癌,Ⅲ～Ⅳ期明显低于Ⅰ～Ⅱ期,伴淋巴结转移者明显低于无淋巴结转移者(P<0.05)。但MMP-1、TI MP-1表达与患者年龄、性别、肿瘤病理类型及肿瘤大小和部位等临床参数无关(P>0.05)。MMP-I与TI MP-1表达呈负相关(r=-0.513,P<0.05)。结论胃癌中MMP-1、TI MP-1表达与胃癌分化程度、TNM分期及淋巴结转移有关。

Objective To investigate the relationship between matrix metalloproteinase-1（MMP-1）,tissue inhibitor of metalloproteinase-1（TIMP-1） and clinic characteristics in gastric cancer.Methods Expressions of MMP-1 and TIMP-1 mRNA were detected in 60 paired samples of primary gastric cancer and tumor-adjacent gastric mucosae by reverse transcriptase-PCR.Results The MMP-1 mRNA expression was higher and TIMP-1 expression was lower in gastric cancers than those in tumor-adjacent gastric mucosae（P〈0.05）.In gastric cancer tissues,the expression of MMP-1 mRNA was higher and TIMP-1 expression was lower in the undifferentiated,grade Ⅲ～Ⅳ,and with lymph node metastasis than those in the well and moderately differentiated,gradeⅠ～Ⅱ,or without lymph node metastasis（P〈0.05）.MMP-1 expression was negatively correlated with TIMP-1 mRNA expression.Expressions of MMP-1 and TIMP-1 mRNA were not significantly correlated with age,gender,histological classification,the size and location of tumors（P〉0.05）.Conclusion The expression of MMP-1 and TIMP-1 in gastric cancer was obviously related with tumor differentiation,stage and lymph node metastases.