肝细胞癌患者外周血单个核细胞端粒酶的表达

Expression of telomerase activity in peripheral blood mononuclear cells in patients with hepatocellular carcinoma

目的探讨外周血单个核细胞(PBMC)端粒酶活性在肝细胞癌(HCC)患者中的意义。方法采用PCR-TRAP-ELISA法检测HCC、慢性肝炎和健康人PBMC端粒酶活性。结果HCC患者PBMC端粒酶活性与肿瘤组织学分化程度、肿瘤直径、血清AFP水平无显著相关性。但与门静脉侵犯和术后1年内复发显著相关。结论PBMC端粒酶活性的高表达是HCC术后复发的指标。

Objective To investigate the telomerase the activity of peripheral blood mononuclear cells（PBMC）in patients with hepatocellular carcinoma.Methods PBMC was isolated from peripheral blood in HCC patients,the patients with chronic liver diseases,and healthy volunteers.Telomerase activity of PBMC was detected using PCR-TRAP-ELISA.Results Telomerse activity of PBMC was markedly increased in HCC patients.There was no significant correlation between the telomerase activity of PBMC and pathological type,tumor diameter or alpha fetus protein（AFP） level,but telomerse activity was closely associated with the portal venous invasion and early postoperative recurrence within one year.Conclusion The expression of telomerase antivity of PBMC may serve as an useful predictor of early postoperative recurrence of HCC.