大孔吸附树脂富集藏药镰形棘豆总黄酮的工艺研究

Study on enriching technic process of total flavones from Herba Oxytropis Falcatae by macroporous resin

目的:建立镰形棘豆总黄酮的大孔吸附树脂富集工艺。方法:二氯氧锆比色法测定样品中总黄酮苷含量,考察D-101大孔吸附树脂富集镰形棘豆水提取物中总黄酮的动态吸附能力,解吸附溶媒浓度及体积,以及树脂的重复利用能力等。结果:富集后总黄酮苷含量达到26%,接近富集前的5倍。结论:D-101型大孔吸附树脂对镰形棘豆水提物中总黄酮成分有较好的吸附能力,60%乙醇溶液解吸附快速有效,树脂重复利用度高。

Objective： To establish an enriching technic process of total flavones from Herba Oxytropis Falcatae by macroporous resin. Methods ： Zirconium oxychloride （ZrOCl2 ） chromatometry was used to determine the content of total flavones in samples. Dynamic state adsorbability, concentration and volume of adsorption stripping solvent, and the re-utilization of D-101 macroporous resin were studied. Results：The content of total flavones in enrichment is 26% , which was nearly five times of that before enriched. Conclusion：Chromatometry with zirconium oxychloride as developer is simple and credible. D-101 macroporous resin showed better adsorbability of flavones from the aqueous extract of Herba Oxytropis Faleatae with high re-availability, and 60% alcohol could be used as adsorption stripping solvent because it works quickly and efficiently.