核因子-κB和nm23-H1在肝细胞肝癌中的表达及其意义

Expression of NF-κB and nm23-H1 protein in hepatocellular carcinoma

目的探讨核因子NF-κB和转移抑制基因nm23-H1在肝细胞癌（HCC）组织的表达情况及与临床病理特征的关系。方法采用免疫组织化学方法检测41例HCC中NF-κB和nm23-H1的表达情况，以相应癌旁肝组织、肝硬化组织（10例）、肝血管瘤旁正常肝组织（11例）作对照，并分析其与病理特征的关系。结果NF-κB蛋白在41例HCC组织中阳性28例，表达率为68．3％；41例相应癌旁肝组织中有5例表达，阳性率12．2％，10例肝硬变组织中1例表达，11例正常肝组织未见表达，HCC与非癌组织中NF-κB蛋白的表达差异有统计学意义（χ^2=41．1，P〈0．01）；NF-κB的表达与HCC的临床病理特点无显著相关性。nm23-H1蛋白在41例HCC组织中14例为阳性染色，27例为阴性染色。nm23-H1阴性表达与肝被膜浸润和门静脉浸润显著相关（χ^2=4．16，χ^2=4．19，P均〈0．05）。结论NF-κB可能参与了HCC的发生、发展，可望作为基因治疗的靶点。nm23-H1在HCC中的阴性表达与HCC的恶性生物学特征相关，可能表明肿瘤的预后不良。

Objective To study the expression of nuclear transcription faetor-KB（NF-κB） and nm23-H1 protein in hepatocellular carcinoma （HCC） and its relationship with clinical pathologic features. Methods The expression of NF-κB and nm23-H1 in 41 cases of HCC were examined by immunohistochemical technique. The corresponding cancerous adjacent tissue or cirrhosis tissue （10 eases）and normal liver tissue （11 cases） collected from specimens of benign liver hemangioma were served as a control group. Results NF-κB protein was detected in 68.3 %（28/41） cases of HCC tissues compared with 12.2 % （5/41） in noncancerous adjacent tissues, 1/10 cases in liver cirrhosis. 0/11 eases in normal liver tissues （P 〈0.01）. NF-κB expression has no correlation with clinical pathological feature of HCC patients fourteen cases of 41 HCC tissues were found nm23-Hl protein positive staining, compared with 27 cases negative staining; negative expression of nm23-H1 protein correlates closely with portal vein invasion and serosal infiltration （χ^2=4.16 χ^2=4.19,P 〈0.05）, and its negative expression correlates well with survivin expression （χ^2= 20.25, P 〈0.01）. Conclusion High expression of NF-κB protein in HCC tissues suggests that it probably played an important rule in the origin and development of HCC, and it may be as targets for gene therapy. Negative expression of nm23-H1 protein correlates with malignant biological feature of HCC, and probably suggests poor prognosis.