摘要
目的运用间接免疫荧光法(IIF)检测抗核抗体(ANA)、抗双链脱氧核糖核酸(抗ds-DNA)及免疫印迹法(IBT)检测抗可提取性抗核抗体(抗ENA)。方法ANA、抗ds-DNA均采用IIF,其中ANA抗原片同时固定有Hep-2细胞和猴肝切片,抗ds-DNA检测抗原片采用绿蝇短膜虫。抗ENA检测采用IBT。共检测自身免疫性疾病患者170例。结果ANA在系统性红斑狼疮(SLE)、干燥综合征、肌炎/皮肌炎、混合性结缔组织病、系统性硬皮病、类风湿关节炎、强直性脊柱炎、过敏性紫癜的阳性率分别为91.6%、100.0%、100.0%、82.4%、65.4%、35.7%、16.7%、42.9%。抗ds-DNA对SLE阳性率为65.1%,特异性为96.6%。抗ENA对自身免疫性疾病(AID)鉴别诊断具有重要意义。结论运用Hep-2/猴肝复合抗原片检测ANA有利于AID的初步筛选和判断。抗ENA抗体谱检测有利于自身免疫疾病的鉴别诊断,抗ds-DNA可作为SLE治疗和监测的指标。
Objective To explore the diagnostic value of combined determination of ANA, anti-dsDNA, anti-ENA polypeptide antibody in autoimmune disease. Methods Totally 170 patients with AID were enrolled in the investigation. Antinuelear antibody （ANA） and ds-DNA were detected with indirect immunofluoreseence assay; anti-ENA polypeptide antibody was detected with immunoblot assay （IBT）. The monkey liver tissue and human epithelial cells （Hep-2） were used as antigen substrate for detection of ANA. The test matrix of ds-DNA was Crithidia luciliae. Results The positive ratesof ANA were 91.6%, 100.0%, 100.0%, 82.4%, 65.4%, 35.7%, 16.7%, and 42.9% in lupus erythematosus （SLE） group, Sjogren＇s syndrome （SS） group, multiple myositis/dermatomyositis （PM/DM） group, mixed connective tissue disease （MCTD） group, progressive systemic scleroderma （PSS） group, rheumatoid arthritis （RA） group, ankylosing spondylitis （AS） group, and allergic purpura （AP） group. The positive rate of anit-ds DNA antibody in SLE group was 65.1 %, and the specificity was 96.6 %. Anti-ENA polypeptide antibody oontributed to the differential diagnosis of AID. Conclusion Detection of ANA with Hep-2/monkey liver tissue as substrate contributes to preliminary screening and identification of AID; detection of anti-ENA antibody repertoire contributes to differential diagnosis of AID; anti-ds DNA antibody may be used a useful marker of curative effect monitoring and prognosis evaluation.
出处
《检验医学与临床》
CAS
2008年第24期1494-1496,共3页
Laboratory Medicine and Clinic
