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IGF-1R的真核表达和单克隆抗体的制备 被引量：2

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摘要 目的:IGF-1R稳定表达株的建立和抗IGF-1R单克隆抗体(mAb)的制备。方法:从cDNA扩增得到人全长IGF-1R基因,构建成含有E-Tag的质粒。用此质粒建立稳定表达IGF-1R的细胞株,并免疫BALB/c小鼠,经融合、筛选制备特异性mAb。结果:成功构建IGF-1R病毒质粒,并筛选得到稳定表达IGF-1R的3T3细胞,该细胞免疫BALB/c小鼠后筛选得到6株稳定分泌抗IGF-1R抗体的杂交瘤细胞株,Western blot结果显示抗体8G3能特异性识别抗原。结论:成功制备了IGF-1R的mAb,为进一步研究IGF-1R在肿瘤治疗中的作用提供了有力的工具。
作者 张翠珍 余君铭 周敏 刘世国 高慧萍 田淑君 李宗海
机构地区 哈尔滨医科大学生理学教研室 上海市肿瘤研究所癌基因及相关基因国家重点实验室
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2008年第12期1192-1193,共2页 Chinese Journal of Cellular and Molecular Immunology
基金 上海市科委重大项目资助(06DZ19501)
关键词 IGF.1R 真核表达 杂交瘤 单克隆抗体
分类号 R392.11 [医药卫生—免疫学]
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参考文献9

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共引文献4

同被引文献22

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细胞与分子免疫学杂志
2008年 第12期

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