Two-step Enzymatic Synthesis of Guanine Arabinoside

Guanine arabinoside (ara-G ) 的化学合成是极其复杂的，费时间，并且严重弄脏。一个二拍子的圆舞酶的合成过程被开发容易获得 ara-G。2,6-Diaminopurine arabinoside (ara-DA ) 首先与嘌呤 nucleoside phosphorylase 和 Enterobacter aerogenes DGW-07 生产的嘧啶 nucleoside phosphorylase 被综合。当反应液体包含了 30 mmol 时， ara-DA 的变换收益能上面到达 90%

The chemical synthesis of Guanine arabinoside （ara-G） is extremely complex, time-consuming, and seriously polluted. A two-step enzymatic synthesis process was developed to acquire ara-G easily. 2,6-Diaminopurine arabinoside （ara-DA） was first synthesized with purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase produced by Enterobacter aerogenes DGW-07. The conversion yield of ara-DA could reach above 90% when the reaction liquid contained 30 mmol·L^-1 uracil arabinoside as arabinose donor, 10 mmol·L^- 1 2,6-diaminopurine as arabinose acceptor in pH 7.0 20 mmol·L^-1 phosphate buffer, and reacted at 60℃ for 48h. Then, ara-DA was effectively transformed into ara-G with adenylate deaminase produced by Aspergillus oryzae DAW-01. The total process had no complex separation and purification.