摘要
Guanine arabinoside (ara-G ) 的化学合成是极其复杂的,费时间,并且严重弄脏。一个二拍子的圆舞酶的合成过程被开发容易获得 ara-G。2,6-Diaminopurine arabinoside (ara-DA ) 首先与嘌呤 nucleoside phosphorylase 和 Enterobacter aerogenes DGW-07 生产的嘧啶 nucleoside phosphorylase 被综合。当反应液体包含了 30 mmol 时, ara-DA 的变换收益能上面到达 90%
The chemical synthesis of Guanine arabinoside (ara-G) is extremely complex, time-consuming, and seriously polluted. A two-step enzymatic synthesis process was developed to acquire ara-G easily. 2,6-Diaminopurine arabinoside (ara-DA) was first synthesized with purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase produced by Enterobacter aerogenes DGW-07. The conversion yield of ara-DA could reach above 90% when the reaction liquid contained 30 mmol·L^-1 uracil arabinoside as arabinose donor, 10 mmol·L^- 1 2,6-diaminopurine as arabinose acceptor in pH 7.0 20 mmol·L^-1 phosphate buffer, and reacted at 60℃ for 48h. Then, ara-DA was effectively transformed into ara-G with adenylate deaminase produced by Aspergillus oryzae DAW-01. The total process had no complex separation and purification.
基金
Supported by the Innovation Fund for Technology Based Firms from Ministry of Science and Technology of China(07C26213101283)
关键词
阿糖鸟苷
合成
两步酶法
生物技术
guanine arabinoside, 2,6-diaminopurine arabinoside, purine nucleoside phosphorylase, pyrimidine nucleoside phosphorylase, adenylate deaminase, Enterobacter aerogenes, Aspergillus oryzae