黄连提取物对肝细胞生长因子受体的特异性抑制作用

Experimental Study on Extract of Huanglian Inhibiting Hepatacyte Growth Factor Receptor Specifically

目的:观察黄连提取物对HepG 2细胞生长的影响,探讨黄连提取物的作用机制。方法:HepG 2细胞培养基中加入肝细胞生长因子,形成过量表达受体酪氨酸激酶(RTK)的HepG 2细胞,加入黄连提取物,测定细胞生长活性、HGFR和P-HGFR的表达水平(活性)。结果:黄连提取物对HepG 2细胞生长、HGFR和P-HGFR的表达水平(活性)均有不同程度的抑制作用。结论:黄连提取物通过抑制RTK而抑制肿瘤细胞的生长。

Objective： To observe extract of Huanglian inhibiting the growth of HepG 2 and explore the mechanism. Methods. HepG 2 cells of excessive expressing receptor tyrasine kinases were established by adding hepatacyte growth factor （HGF） to MEM medium. Expressions of HGFR and P- HGFR were detected when supplemented extract of Huanglian or not. Results. Extract of Huanglian inhibited the growth of HepG 2, the expression of HGFR and the activity of HGFR to ,some extent. Conclusion： Extract of Huanglian inhibited the growth of HepG 2 by down - regulating expressions of HGFR and p- HGFR.