摘要
目的通过观察重组人红细胞生成素(recombinant human erythropoietin,rHu-EPO)对大鼠急性脊髓损伤后Caspase-3表达及凋亡的影响,探讨其脊髓保护的作用机制。方法60只SD大鼠随机分成3组:假手术组,对照组和治疗组,每组20只,采用Allen's打击法制成急性脊髓损伤模型。观察药物治疗前后大鼠的神经功能行为,用免疫组化染色检测caspase-3表达,原位脱氧糖核苷酸末端转移酶介导的缺口末端标记法(Tunel法)标记凋亡细胞;比较各组间差别。结果HE,免疫组化染色示EPO治疗组脊髓损伤程度小,神经元细胞破坏少;caspase-3在各时相点的表达明显降低,8h和7dTunel标记凋亡阳性细胞显著减少;大鼠神经功能恢复显著优于对照组(<0.01)。结论EPO能下调Caspase-3表达,抑制脊髓神经细胞凋亡,对继发性脊髓损伤有保护作用。
Objective To investigate the effect of rH-EPO on expressional level ofcaspase-3 and apoptosis after spinal cord injury, explore its protective effect and action mechanism. Methods 60 SD rats were divided into three groups by randomization： Sham, Control, EPO cure groups. There were 20 rats in each group. SD rat model of acute spinal cord injury were established by Allen＇s weight dropping （WD） .The change of nerve functions, the expression ofcaspase-3 was tested with immunohistochemistry and the apoptosis was labeled by Tunel. The differences and effects of each group were compared. Result HE staining pathological changes were mitigated than control group. The caspase-3 protein level was lower than that in control group. Tunel-positive cells were significantly decreased at 8h and 7d; the grade of nerve functions was improved distinctly（P〈0.01）. Conclusion EPO can decrease the expression ofcaspase-3 and inhibit neuronal and glial cell apoptosis in the rats after spinal cord injury, which may play a significant neuroprotective role in the secondary SCI.
出处
《生物骨科材料与临床研究》
CAS
2008年第6期14-16,21,共4页
Orthopaedic Biomechanics Materials and Clinical Study
