玉米幼胚培养及植株再生的研究

Studied on Tissue Culture and Plant Regeneration of Maize Immature Embryos

以不同玉米自交系幼胚为外植体,研究不同2,4-D浓度对愈伤组织诱导、6-BA浓度对愈伤组织分化和6-BA与NAA配合使用对试管苗生根的影响。结果表明:不同2,4-D浓度对愈伤组织诱导率影响差异不明显,但对胚性率诱导有明显影响,其诱导最佳培养基为MS+2,4-D 2 mg/L+水解酪蛋白500 mg/L+脯氨酸500 mg/L;愈伤组织分化的最佳培养基是MS+6-BA0.5 mg/L+水解酪蛋白500 mg/L+谷氨酰胺200 mg/L+PP333 2 mg/L;试管苗生根的最佳培养基是1/2 MS+6-BA0.5 mg/L+NAA2.0 mg/L+PP333 2.0 mg/L+活性炭0.2%。

The inducement, differentiation and plantlet roots of callus were studied from immature embryos of the different maize inbred lines in vitro on different medium with the different 2,4-D concentration. The results showed that the inducement rate of callus was not significant with the different 2,4-D concentration, but the rate of inducement of EC was significant, and the medium was MS ＋ 2,4-D 2 mg/L ＋ CH 500 mg/L ＋ Pro 500 mg/L. The differentiation of callus was best on the culture medium of 1/2 MS ＋ 6-BA 0.5 mg/L ＋ NAA 2.0 mg/L ＋ PP333 2.0 mg/L＋ active carbon MS ＋ 6-BA 0.5 mg/L ＋ CH 500 mg/L ＋ GLU 200 mg/L ＋ PP333 2 mg/L. The plantlet rooting was best on the culture medium of 1/2 MS ＋ 6-BA 0.5 mg/L ＋ NAA 2.0 mg/L ＋ PP333 2.0 mg/L ＋ active carbon 0.2%.