6省区玉米丝黑穗病菌遗传多样性分析

Analysis on Genetic Diversity of Sporisorium reilianum from 6 Provinces and Regions in China

采用随机扩增多态性DNA技术(RAPD)对我国6省区玉米丝黑穗病菌的生理分化情况进行了初步研究。通过10个随机引物将来自于吉林、辽宁、黑龙江、山西、河北、内蒙古自治区的102株玉米丝黑穗病菌大致划分为6个类群(相似系数0.42),在更高的相似水平上,类群A和类群B还可以进一步划分为众多的亚群。从聚类分析的结果可以看出,相似性较高的菌株大多来源于相同或相近的地区,说明玉米丝黑穗病菌的亲缘关系存在一定的地域性。

One hundred and two strains of S. reilianum from six provinces and regions （Jilin,Liaoning,Heilongjiang, Hebei, Shanxi, Inner Mongolia） were divided into six pathogenic groups by ten primers with RAPD anylysis （percent agreement 0.42）. Group A and Group B were further divided into more subgroup at high simi-arity level. The results indicated that there was rich significance for strains of S. reilianum, but high similarity strains were from same or near areas, genetic diversity of S. reilianum were relation to the areas.