草莓愈伤组织诱导的几个影响因素研究

Study on Factors of Affecting Callus Induction of Strawberry

在MS培养基上附加不同种类与浓度的外源激素,以草莓品种大将军和香妃的花药和叶片为外植体,对影响草莓愈伤组织诱导的因素进行了研究。结果表明,愈伤组织的出愈率除受激素浓度和配比的影响外,还受品种基因型和外植体的影响。MS+0.5 mg/L 6-BA+0.25 mg/L 2,4-D适于作大将军花药和叶片愈伤组织诱导的培养基,其出愈率分别达48.58%和39.89%;MS+2.0 mg/L 6-BA+1.0 mg/L 2,4-D适用于香妃花药和叶片愈伤组织的诱导,其出愈率分别达42.13%和35.30%。在同一种培养基上,大将军愈伤组织的出愈率比香妃高,且两品种花药愈伤组织的出愈率均高于叶片的出愈率。

The factors of genotpye, concentration of growth regulators and explants that affecting callus induction of strawberry were studied. The result showed that the optimum medium for callus induction from anther and leave of Da-jiangjun was the medium supplemented with MS＋0. 5 mg/L＋6-BA＋0. 25 mg/L 2,4-D, with the induction rate up to 48.58% and 39.89%, respectively. The medium of supplemented with MS＋2.0 mg/L 6-BA＋1.0mg/L 2,4-D was suitable for callus induction of Xiangfei, the induction rate from anther and leave was 42. 13% and 35. 30%. In the same medium, the callus-induction rate of Dajiangjun was higher than of Xiangfei. The anther callus-induction rate of two varieties was higher than of the leave.