猪细小病毒的PCR检测、分离及鉴定

PCR Detection,Isolation and Identification of Porcine Parvovirus

根据GenBank中报道的猪细小病毒基因组序列,利用PCR引物设计软件,设计并合成了1对引物,通过对PCR反应条件的优化,成功地扩增出了511bp的目的片段,建立了PCR检测PPV的方法。利用该方法对120份临床样品进行检测,共检测到4份阳性样品,并成功地分离到2株PPV,1#病毒分离株理化特性鉴定表明其符合PPV的特性。

Based on the gene sequences of porcine parvovirus （PPV） in GenBank, a pair of primers was designed and synthesized. Expected fragment was successfully amplified by PCR, which was about 511 base pair. Four positive samples out of 120 clinical samples were detected with this technique. Two strains of PPV were success-fully isolated by cell culture. The physico - chemical property of No. 1 strain was consistent with the feature of PPV.