大肠癌中MT_1-MMP和RECK基因表达的研究

STUDY ON THE EXPRESSIONS OF MEMBRANE TYPE-1 MATRIX METALLOPROTEINASE AND RECK GENE IN COLORECTAL CANCER

目的探讨膜型基质金属蛋白酶-1(MT1-MMP)和RECK基因在大肠癌中的表达及其临床病理意义。方法采用免疫组织化学SP法检测216例大肠癌组织及20例正常大肠黏膜组织中MT1-MMP与RECK的表达,并分析它们的表达与大肠癌临床病理因素的关系。结果MT1-MMP蛋白在216例大肠癌组织中的阳性表达率为81.5%,在20例大肠正常黏膜中的阳性表达率为0(P<0.01);在Ⅰ+Ⅱ与Ⅲ+Ⅳ期的阳性表达率分别为67.1%和88.4%(P<0.01);在T1+T2与T3+T4两组的阳性表达率分别为72.5%和88.0%(P<0.05);在N0与N1+N2两组的阳性表达率分别为65.7%和89.0%(P<0.01);在M0与M1两组的阳性表达率分别为77.7%和94.0%(P<0.01)。RECK在216例大肠癌组织中的阴性表达率为41.7%,在20例大肠正常黏膜中的阴性表达率为0(P<0.01);在Ⅰ+Ⅱ与Ⅲ+Ⅳ期的阴性表达率分别为21.4%和51.4%(P<0.01);在T1+T2与T3+T4两组的阴性表达率分别为26.4%和52.8%(P<0.01);在N0与N1+N2两组的阴性表达率分别为25.7%和49.3%(P<0.01);在M0与M1两组的阴性表达率为34.3%和66.0%(P<0.01);RECK阴性表达组(5年生存率为25.6%)较阳性表达组(5年生存率为52.4%)预后差(P<0.01)。结论MT1-MMP蛋白在大肠癌中的表达与肿瘤分期、淋巴结转移、器官转移及预后有关,在大肠癌的侵袭、转移中发挥重要作用。

Objective To Study the expression of membrane Type-1 matrix metalloproteinase（MT1-MMP） and RECK gene and their clinical significance in colorectal cancer.Methods The expressions of MT1-MMP protein and RECK in 216 cases of colorectal cancer were detected by immunohistochemistry assay and their correlations with clinicopathological factors were analyzed.Results Of 216 cases of colorectal cancer,the positive MT1-MMP expression rate was 81.5%,and that was 0% of 20 normal tissues（P〈0.01）.The positive rates were 67.1% and 88.4% in stage Ⅰ＋Ⅱ and Ⅲ＋Ⅳ,respectively（P〈0.01）,72.5% and 88.0% in the group of T1＋T2 and T3＋T4（P〈0.01）,65.7% and 89.0% in the group of N0 and N1＋N2（P〈0.01）,and 77.7% and 94.0% in the group of M0 and M1（P〈0.01）.Of 216 colorectal cancer,the RECK negative-expression rate was 41.7%,and that was 0% of 20 normal tissues（P〈0.01）.The negative-expression rates were 21.4% and 51.4% in stage Ⅰ＋Ⅱ and Ⅲ＋Ⅳ,respectively（P〈0.01）,26.4% and 52.8% in the group of T1＋T2 and T3＋T4（P〈0.01）,25.7% and 49.3% in the group of N0 and N1＋N2（P〈0.01）,and 34.3% and 66.0% in the group of M0 and M1（P〈0.01）.The RECK negative-expression group（5-year overall survival rate：25.6%） had a worse prognosis than that of high-expression group（5-year overall survival rate：52.4%）（P〈0.01）.Conclusion MT1-MMP protein and RECK gene expressions in colorectal cancer have positive relation to the stage,lymph-node metastasis,distant metastasis and the prognosis of patients.MT1-MMP protein and RECK gene expression may play a key role in the invasion and metastasis of colorectal cancer.