二巯基丙磺酸钠对食管癌EC109细胞系和小鼠艾氏腹水癌的作用研究

AN INVESTIGATION ON THE EFFECTS OF SODIUM DIMERCAPTOPROPANSULFONATE ON ESOPHAGEAL CANCER CELL LINE EC109 IN VITRO AND ON MICE BEARING EHRLICH ASCITES CANCER

目的观察二巯基丙磺酸钠(DMPS)对食管癌EC-109细胞和小鼠艾氏腹水癌的作用。方法在体外培养的食管癌EC-109细胞株中,通过MTT实验,观察不同浓度的DMPS对肿瘤细胞生长增殖的抑制作用;通过荧光显微镜和细胞凋亡-Hoechst33258染色试剂盒,观察不同浓度的DMPS对肿瘤细胞凋亡的诱导作用。在小鼠艾氏腹水癌中,观察不同浓度的DMPS对小鼠生存期的影响。结果体外实验中,终浓度为8、4、21、mmol/L的DMPS应用24 h和48 h后,对肿瘤细胞的生长增殖有显著地促进作用(P<0.01);应用72 h后,则显著地抑制肿瘤细胞的生长增殖(P<0.01)。终浓度为8、4、2、1 mmol/L的DMPS应用24 h和48 h后,肿瘤细胞凋亡数量无显著改变(P>0.05),但应用72 h后,各浓度组可显著增加肿瘤细胞凋亡数量(P<0.01)。体内实验中,751、50 mg/kg的DMPS腹腔注射,每天1次,连续10 d,可显著地延长小鼠生存时间(P<0.01)。结论DMPS应用72 h后,可能通过诱导肿瘤细胞的凋亡而显著地抑制体外食管癌EC109细胞系的生长增殖;腹腔注射751、50 mg/kg连续10 d,可延长艾氏腹水癌小鼠的生存时间。

Objective To investigate the effects of sodium dimercaptopropansulfonate（DMPS） on esophageal cancer cell line EC109 in vitro and on mice bearing Ehrlich ascites cancer.Methods The esophageal cancer cell line EC109 cultured in vitro was applied to test the effects of DMPS with different concentrations on the growth and proliferation of the cancer cells by means of MTT method,and also to test the apoptosis induction of DMPS with different concentrations via fluorescence microscope and with cell apoptosisHoechst33258 kit,the effects of DMPS with different concentrations on the survival time of mice bearing Ehrlich ascites cancer was investigated.Results In vitro,DMPS,at the final concentrations of 8,4,2 and 1mmol/L,significantly stimulated EC109 cells growth and proliferation when administered 24h（P〈0.01） and 48h（P〈0.01） later,respectively,while 72h after administration,all concentrations of DMPS significantly inhibited the growth and proliferation of EC109 cell（P〈0.01）.DMPS,at the final concentration of 8,4,2 and 1mmol/L,had no significant effects on the number of apoptotic EC109 cells when administered 24h and 48h later,respectively（P〉0.05）.However,all concentrations of DMPS significantly increased the number of apoptotic EC109 cells when administered 72h later（P〈0.01）.In vivo,DMPS administered daily intraperitoneally at a dose of 75mg/kg body weight and 150mg/kg body weight for 10 days,significantly lengthened the survival time of mice bearing Ehrlich ascites cancer（P〈0.01）.Conclusion DMPS significantly suppresses the growth and proliferation of esophageal cancer cell line EC109 in vitro 72h after administration,possibly due to induction of apoptosis,and DMPS daily administered intraperitonealy for 10 days could lengthen the survival time of mice bearing Ehrlich ascites cancer.