摘要
目的评价化学发光法检测梅毒螺旋体特异性抗体的效果。方法5家实验室分别用酶联免疫吸附试验(ELISA)、化学发光检测法(CLIA)和梅毒螺旋体明胶颗粒凝胶试验(TPPA)进行临床标本的双盲检测。以TPPA结果为标准,分析其敏感性和特异性。结果共计检测1525例血清样品,其中TPPA确证阳性308例,阳性率为20.2%。以TPPA作参照,发光法敏感性为100%(308/308),特异性为99.3%(1208/1217),阳性结果预测值(PPV)为97.2%(308/317),阴性结果预测值(NPV)为100%(1208/1208);5种ELISA试剂总体敏感性为98.1%(302/308),特异性为97.9%(1192/1217),PPV为92.4%(302/327),NPV为99.5%(1192/1198)。结论CLIA法具有敏感性高和特异性强的特点,适合于临床实验室进行梅毒的筛查。
Objective To evaluate the effect of chemiluminescence immunoassay （CLIA） for detecting the antibody to Treponema palledum. Methods The evaluation was carried out by double blind experiments with ELISA, CLIA and TPPA in five different laboratories. Compared with the results of TPPA as a reference, the specificity and sensitivity were analyzed. Results Out of 1 525 sera detected, 308 were positive by TPPA,accounting for 20.2 %. Compared with the results of TPPA, the sensitivity,specificity,positive predictive value （PPV） and negative predictive value （NPV） of CLIA were 100% （308/308）,99.3% （1 208/1 217）,97.2% （308/317） and 100% （1 208/1 208）,respectively,while those of ELISA were 98.1% （302/308）,97.9% （1 192/1 217）,92.4% （302/327）and 99.5% （1 192/1 198）,respectively. Conclusion CLIA is characterized with higher sensitivity and specificity. It is suitable for screening Syphilis in clinical laboratory.
出处
《中国艾滋病性病》
CAS
2008年第6期609-610,616,共3页
Chinese Journal of Aids & STD
关键词
梅毒
抗体
化学发光
评价
Syphilis
Antibody
Chemiluminescence Immunoassay
Evaluation
