肌萎缩侧索硬化与血浆同型半胱氨酸关系的研究

Relationship between plasma level of homocysteine and amyotrophic lateral sclerosis

目的探讨肌萎缩侧索硬化与同型半胱氨酸水平的关系。方法采用断面研究,21例肌萎缩侧索硬化患者为ALS组,选取40例健康者为对照组,检测两组患者的Hcy、叶酸、维生素B_(12),用高效液相色谱仪和荧光检测仪测定两组血浆总Hcy水平。结果 Hcy水平:肌萎缩侧索硬化组[(17.65±11.23)μmol/L]显著高于对照组[(10.32±6.72)μmol/L]。肌萎缩侧索硬化组叶酸水平[(9.52±2.57)ng/ml]明显低于对照组[(13.56±3.23)ng/ml]。维生素B_(12)水平在两组间无统计学差异(P>0.05),肌萎缩侧索硬化组为(651±112.34)pg/ml,对照组为(632±164.89)pg/ml。结论 Hcy水平升高与肌萎缩侧索硬化密切相关,可能参与了肌萎缩侧索硬化的病理生理过程。

Objective To examine tile relationship between Hcy levels and clinical ALS. Methods In a cross -sectional study, 21 amyotrophic lateral sclerosis patients were enrolled as ALS group and 40 healthy person as control group. The plasma levels of Hcy, B12, and folate levels were measured in all subjects. Hcy levels were measured using high performance liquid chromatography with fluorescence detection. Results Patients with ALS had higher median plasma Hcy levels [ 17.65 （ 17.65 ± 11.23） vs 10. 32 （ 10. 32 ±6.72） umol/L; P 〈0. 01 ] and lower folate levels [9. 52 （9. 52 ±2. 57） vs 13. 56 （ 13. 56 ±3.23） ng/ml; P 〈 0.01 ]. Compared with controls, no significant difference was observed in plasma Bl2 levels between the two groups, （651 ± 112. 34）pg/ml in ALS group, （632 ± 164. 89）pg/ml in control group. Conclusion High plasma homocysteine （Hey） level was related with amyotrophic lateral sclerosis （ALS） compared with healthy controls, and may be linked to pathophysiologic progression of the disease.