水稻同核异质雄性不育系的选育研究

Study on breeding of homokaryoticheteroplasmic malesterile lines in rice

利用海南野败质型、红莲质型、日本ＢＴ质型、滇型、田东野稻质型、隆安野稻质型、Ｄｉｓｉ质型、冈比亚卡质型、印尼水田谷质型、矮秆野败质型、Ｋ型和马协不育系等１２种不同细胞质来源的雄性不育系，同１８个常规品种杂交，并经连续回交选育，于Ｂ７Ｆ１选育出了粳选２号和粳选６号细胞核和不同细胞质相结合的同核异质雄性不育系稳定系统２４个。研究结果表明，粳选２号和粳选６号同核异质雄性不育系中典败花粉型、圆败花粉型和染败花粉型细胞质不育因子的表达，可能分别受核内相对应基因调控，属于多种核质基因对应性互作关系。

Twelve cytoplasmic malesterile(CMS)lines with the cytoplasm belonging to Hainan wild rice abortion(WA) type, Honglien type, BT type, DianYi type, TianDong wild rice type, NongAn wild rice type, Dissi type, Gambiaka type ,Indonesia rice type, dwarf WA type, K type and MaXie CMS lines were crossed to 18 rice varieties. After continuously selecting and backcrossing, two sets of homokaryoticheteroplasmic CMS lines named Japonica rice No.2 and Japonica rice No.6 were developed in B7F1. Experiment results showed that the expression of the cytoplasmic malesterile factors for the typical abortion type, spherical abortion type and stained abortion type in these homokaryoticheteroplasmic lines were controlled by the corresponding nuclear genes. The model of multiple nucleocytoplasmic gene corresponding interaction is applicable to these lines.