高压氧对大鼠脊髓损伤后脂质氧化过程的影响

Influence of lipid oxidation process for spinal cord injury in rats after hyperbaric oxygen

目的观察大鼠脊髓损伤后高压氧(HBO)对脊髓损伤(SCI)又氧自由基引起的脂质氧化过程和功能的影响,探讨高压氧对脊髓继发性损伤的保护作用。方法SD大鼠30只随机分为3组,一组椎板切除不损伤脊髓,另两组采用改良Allen法制备大鼠SCI模型,一组术后两小时内进行高压氧治疗,治疗时间共90分钟,1天1次,连续进行5天;另一组不进行任何处理。分别于术后第2、5天尾静脉取血,测定血清中超氧化物歧化酶(SOD)、脂质过氧化产物丙二醛(MDA)。结果高压氧组与非高压氧组相比较,高压氧组血清中SOD升高(P<0.01),MDA降低(P<0.01),与假手术组相比,SOD和MDA变化不明显,无统计学意义(P>0.01)。结论高压氧具有抗氧化、清除自由基功能,能有效抑制SCI受损引起脂质过氧化反应,对SCI具有保护和治疗作用,并能促进神经功能的恢复。

Objective To observe the spinal cord injury in rats after hyperbaric oxygen （HBO） for spinal cord injury （SCI） caused by oxygen free radicals and oxidation of lipid oxidation and function, to explore the high-pressure oxygen on the secondary spinal cord injury in rats. Methods 30 SD rats were randomly divided into 3 groups, a group of non-laminectomy spinal cord injury and the other two modified Allen prepared rat SCI model, a group for two hours after hyperbaric oxygen therapy, the treatment time of 90 minutes, the first day in a row for 5 days; the other group did not deal with. With the next day after, on the fifth day, the tail vein blood, serum superoxide dismutase （SOD）, Malondialdehyde （MDA）. Results Hyperbaric oxygen group and the results of the non-hyperbaric oxygen group compared with hyperbaric oxygen group after the serum SOD increased （P〈0.01）, MDA reduced （P〈0.01）, and Sham group compared to MDA and SOD did not change significantly, there was no statistical significance （P〉0.01）. Conclusion Of the high-pressure oxygen free radicals have a clear anti-oxidation function of SCI to effectively curb damage caused by lipid peroxidation, SCI has a role in the protection and treatment, and the promotion of neurological recovery.