中药穴位敷贴疗法对哮喘大鼠炎症反应的影响

Effect on Acupoint External Application with Chinese Herbal Medicine on Inflammatory Reaction in Asthma Rats

【目的】观察穴位敷贴疗法对哮喘大鼠炎症反应的影响。【方法】选用Wistar大鼠33只随机分为正常组、模型组、地塞米松组及穴位贴药组;模型组以卵蛋白致敏并诱发哮喘模型,腹腔注射生理盐水,同时背部给予空白对照药物敷贴;地塞米松组在哮喘大鼠雾化后予以腹腔注射1 mg/kg地塞米松,同时在背部备皮予以空白对照药物敷贴;穴位贴药组在哮喘大鼠雾化后予以腹腔注射1 mg/kg的生理盐水,同时在背部备皮予以治疗药物敷贴,穴位取大椎、肺俞、脾俞、肾俞穴。疗程结束24 h内取各组大鼠肺组织观察其炎症情况,并取外周血采用酶联免疫吸附(ELISA)法比较各组大鼠白细胞介素4(IL-4)、干扰素γ(IFN-γ)的含量。【结果】模型组大鼠与正常组比较,肺组织中可见嗜酸性粒细胞(EOS)、淋巴细胞及巨噬细胞浸润增加(P<0.01)。地塞米松组与模型组比较嗜酸性粒细胞、淋巴细胞及巨噬细胞浸润显著降低(P<0.05或P<0.01)。穴位贴药组对嗜酸性粒细胞浸润也有显著抑制作用(P<0.01),但对于淋巴细胞及巨噬细胞浸润无显著抑制作用(P>0.05)。模型组大鼠外周血IL-4含量较正常组显著增加(P<0.01),而IFN-γ含量较正常组显著降低(P<0.01),地塞米松组及穴位贴药组中大鼠外周血IL-4含量较模型组降低(P<0.01),但地塞米松组大鼠外周血IFN-γ含量与模型组比较无显著性差异(P>0.05),而穴位贴药组大鼠外周血IFN-γ含量显著高于模型组(P<0.01),与正常组比较无显著性差异(P>0.05)。【结论】中药穴位敷贴治疗哮喘的作用可能与其能抑制肺组织嗜酸性粒细胞浸润,降低外周血IL-4含量,升高IFN-γ含量有关。

Objective inflammatory reaction in To observe the effect of asthma rats. Methods acupoint external application （AEP） of Chinese herbal medicine on A total of 33 Wistar rats were 6） , model group （N = 9）, dexamethasone group （ DXM established by intraperitoneal injection of 10% ovalbumin randomized into normal group （N = N = 9 ） and AEP group （N = 9 ）. Asthma model was （OVA） 1 mL and spray inhalation of 2% OVA 25 mL for 40 min, once daily for 2 weeks. The acupoints of Dazhui （GV14） , Pishu （BI20） , Feishu （ BL13 ） and Shenshu （BL23） were selected for external application of Chinese herbal medicine. The model group received intraperitoneal injection of normal saline and AEP of blank control Chinese herbal medicine, DXM group received intraperitoneal injection of DXM 1 mg/kg and AEP of blank control Chinese herbal medicine after spray inhalation, and AEP group received intraperitoneal injection of normal saline 1 mg/kg and AEP of Chinese herbal medicine after spray inhalation. The inflammation of the lung tissue was examined, and peripheral blood contents of interleukin-4 （IL-4） and interferon γ （IFN-γ） were detected by enzyme-linked immunosorbent assay （ELISA） 24 hours after the treatment. Results The infiltration of eosinophils （EOS）, lymphocytes and macrophages was increased obviously in the model group （ P 〈 0. 01 compared with that in the normal group）, and was decreased in DXM group as compared with the model group （ P 〈 0.01 or P 〈 0. 01 ）. The infiltration of EOS in AEP group was inhibited obviously （ P 〈 0. 01 ）, but the infihration of lymphocytes and macrophages remained unchanged as compared with the model group （P 〉0. 05 ）. The peripheral IL-4 content was obviously increased （P 〈0. 01 ）, and IFN-γ decreased in the model group as compared with the normal group （P 〈0.01 ）. IL-4 content was decreased in DXM group and AEP group （P 〈0. 01 compared with the model group）, and IFN-γin AEP group was higher than that in the model group （P 〈 0. 01 ）, and arrived to the level in the normal group （ P 〉 0. 05 ）. However, the difference of IFN-γ between DXM group and the model group was insignificant （ P 〉 0.05 ）. Conclusion The therapeutic mechanism of AEP of Chinese herbal medicine for asthma may be related with the inhibition of EOS infiltration, the decrease of peripheral IL-4 content and the increase of IFN-γ.