左旋咪唑对新城疫灭活疫苗抗体滴度的影响

Effect of Levamisole on Antibody titer of ND inactive vaccine

为探讨免疫增强剂左旋咪唑对新城疫灭活疫苗抗体滴度的影响,将20只20日龄雏鸡,随机分成A、B、C、D4组,每组5只。分别皮下注射新城疫灭活疫苗0.5ml/羽,同时注射A组雏鸡高(0.6mg)、B组中(0.3mg)、C组低(0.15mg)剂量的左旋咪唑,对照组D不加。免疫后0天、7天、14天、21天和28天,翅下静脉采血并检测新城疫抗体滴度。结果显示:免疫前4组抗体水平基本一致,无统计学意义;免疫后7天,14天,21天,高、中、低3组抗体水平基本一致,4组的抗体水平相差均在2个滴度以内,统计分析无明显差异;免疫后28天,B组抗体水平较高。统计分析显示,对照组与高、中、低3组均有显著差异,但无剂量差异;在高、中、低3组中,以中剂量添加最佳,抗体效价高(7.2log2),但中剂量组与高、低组的差异不显著。这表明,左旋咪唑的添加提高新城疫抗体滴度,并且与免疫后时间长短有关系。从趋势看,随着时间的推移,添加组的抗体水平好于对照组。

To investigate the effect of levamisole on antibody tiger of ND vaccine,the testing chicken was divided into four groups named group A、B、C and D,5 chick/group.A、B、C group were treated with Levamisole and Newcastle disease vaccine.A group was high dose including 0.6mg Levamisole,B middle dose 0.3 mg,C low dose 0.15 mg,and D control.The blood samples were collected at 7 d,14 d,21 d,28 d after inoculation,and analyzed with hem-agglutination inhibition（HI）.The results showed that the antibody titer of the four group was of no difference before immune.The antibody titer among high,middle,and low dose group was not different statistically at the 7 d,14 d and 21d after immune.The antibody titer of the middle dose group was higher at 28 d after immune.Statistics and analyses proved that the control group was different from other 3 groups,which implied that the groups treated with Levamisole were greatly significant.However it is not relative to dose of Levamisole.B middle dose group displayed the best performance among A、B、C group,and the antibody value was high（7.2log2）,but the difference was not remarkable compared with A、C group.This indicated that addiction of Levamisole in ND vaccine could promote antibody titer,and titer tendency was upward along with time.