不同倍性栝楼生长发育及果实糖酸含量的研究

Studies on the Growth and Sugar/Acid Contents between Autotetraploid and Diploid of Trichosanthes Kirilowli Mamm

为了进一步培育栝楼优良品种,也为药用植物多倍体诱变育种提供依据,以天然二倍体和笔者所在课题组人工诱变成的四倍体栝楼为材料,对其生长发育和果实中总糖和总酸含量进行测定研究。利用蒽酮法和酸碱滴定法测定天然二倍体和四倍体栝楼果实中的糖和酸含量。研究结果表明:四倍体栝楼的长势快于二倍体;叶片中叶绿素含量明显高于二倍体;四倍体栝楼果实大小、重量、结实率和百粒重明显小于二倍体;果实中总糖和总酸的含量四倍体明显高于二倍体。由DPS软件分析可知:在0.05水平下,四倍体和二倍体栝楼总糖和总酸的含量有显著的差异;在0.01水平下,总糖和总酸的含量有极显著的差异。

In this paper,the growth and sugar/acid contents were studied between our obtained autotetraploid and diploid of Trichosanthes Kirilowli Mamm for the breeding of new herb cultivar.The anthrone method and acid-base titration were used to mensurate sugar/acid contents between autotetraploid and diploid of Trichosanthes Kirilowli Mamm.It showed that the growth vigor of tetraploid is faster than diploid,chlorophyll content in leaf of tetraploid was higher than that in diploid.The°fruit size and weight,seed setting rate and the weight of 100 seeds of tetraploid were all much lower than diploid.Sugar content and acid content in tetraploid fruits were all higher than that in diploid fruits.By statistical analysis of DPS software,it showed that the differences of sugar content and acid content in tetraploid fruits were significant at 0.01 level and significant at 0.05 level when comparing with that in diploid fruits.