运用随机扩增多态性DNA标记检测中国东北大豆灰斑病菌株遗传变异

USE OF RANDOM AMPLIFIED POLYMORPHIC DNA MARKERS FOR THE DETECTION OF GENETIC VARIATION IN CERCOSPORIDIUM SOJINUM OF NORTHEAST CHINA

运用致病力和ＤＮＡ多态性检测中国东北地区的３５个大豆灰斑病菌分离物的遗传变异、根据菌株在９个品种（系）上的致病力反应可将其分为７个组。利用１３个随机引物扩增供试菌株共计产生１０５个ＲＡＰＤ标记，其中７８．１％具有多态性。通过聚类分析计算了各菌株间的遗传距离，并产生树状图，发现同一地区内及不同地区间的病菌表现遗传变异、致病性和ＤＮＡ多态性间具有一定相关性。

Inoculation tests on soybean cultivars (or lines) and random amplified polymorphic DNA markers were used to assess pathogenicity and genetic diversity among 35 isolates of Cercosporidium sojinum collected from Northeast region in China. Based on Pathogenicity tests on nine differential soybean cultivars and lines, isolates were differentiated into 6 groups. Of 105 RAPD loci which amplified with 13 random decamer primers, 78.1% were polymorphisms. Genetic distances between each of the isolates were calculated, and cluster analysis were used to generated dendrogram showing relationships between them. The results showed that genetic diversity were existed among isolates from same location as well as different location. The relationship between pathogenicity and RAPD markers were presented in some isolates of C. sojinum .