用PhastSystem 电泳仪快速鉴定根结线虫种类

USING PHASTSYSTEM FOR RAPID IDENTIFICATION OF ROOT KNOT NEMATODES

应用瑞典ＰｈａｒｍａｃｉａＢｉｏｔｅｃｈ．公司开发的全自动快速水平电泳仪（ＰｈａｓｔＳｙｓｔｅｍ）进行酯酶和苹果酸脱氢酶电泳，对云南省烟草和观赏植物一串红上的根结线虫进行快速鉴定，得出４种根结线虫：花生根结线虫（Ｍｅｌｏｉｄｏｇｙｎｅａｒｅｎａｒｉａ）、爪哇根结线虫（Ｍ．ｊａｖａｎｉｃａ）、南方根结线虫（Ｍ．ｉｎｃｏｇｎｉｔａ）和北方根结线虫（Ｍ．ｈａｐｌａ）。文章提供电泳方法，认为ＰｈａｓｔＳｙｓｔｅｍ电泳仪是根结线虫种类快速鉴定的理想设备。

Electrophoresis of esterase and malate dehydrogenase(MDH) was conducted using Phast System(Pharmacia Biotech.) for the rapid identification of root knot nematodes on Nicotiana tabacum and Salvia splendeus in Yunnan.Four species were used in the experiment,ie. Meloidogyne arenaria,M.javanica,M.incognita, and M.hapla. The procedure of electrophoresis using PhastSystem is provided here.The experiment demonstrated that PhastSystem is an ideal instrument for rapid identificaion of root knot nematode species.