摘要
从组织细胞制备、不同试验细胞（ｐｋ１５，ＳＴ）及不同维持液组成成份三个方面对ＴＧＥＶ在组织细胞上增殖情况进行了比较。结果表明，用含１５％ＦＢＳ培养液进行ＳＴ或ｐｋ１５细胞传代并制备悬液，操作方便，能形成良好单层，利用ＴＧＥＶＣＰＥ判定；ＳＴ细胞在病毒增殖和检测毒价两方面均较ｐｋ１５细胞分别高２个滴度和１个滴度以上；维护液中提高血清含量（８％ＦＢＳ）或含一定量胰酶对ＴＧＥＶ增殖效果影响不大。
In this paper we compared the roles of tissue culture preparation,different test cell lines(pk15,ST)and maintenance medium copmosition in TGEV propagation in tissue cultures.The results showed that ST or pk15 passage and cell suspension preparation using 15% FBS growth medium facilitated good monolayer formation and TGEV CPE confirmation ST cell line was more sensitive both in TGEV propagation and virus titration than pk15 cell line,by more than 2 titres and 1 titre higher respectively,Maintenance media using higher FBS rate(8%)or with a small rate of trypsin had no obvions influences on TGEV propagation.
关键词
传染性胃肠炎
病毒
细胞培养
病毒增殖
猪病
Swine transmissible gastroenteritis virus(TGEV) Tissue cultures(ST,pk15) Virus propagation Virus titration
