Dot-ELISA检测减蛋综合征病毒的研究

Detection of Egg Drop Syndrome Virus by DotELISA

将辣根过氧化物酶（ＨＲＰ）直接标记到ＭｃＡｂ（ＩｇＧ）上，建立了检测减蛋综合征（ＥＤＳ７６）病毒的ＤｏｔＥＬＩＳＡ方法，可敏感、特异、及早、快速地检测细胞培养物和实验感染鸡样品中的病毒抗原，某发病鸡场的泄殖腔拭子样品的阳性率为４３３３％（１３／３０）。

The DotELISA to detect DES76 virus was developed by using HRPlabelled McAb(IgG).The EDS76 virus in cell cultures and the samples from experimental infected birds could be detected sensitively,specifically and quickly.The positive rate of cloaca swabs from one chicken farm was 4333%(13/30) 