葡萄脯氨酸累积变异系CAT和SOD活性研究

CAT and SOD Activities of Grape Proline Accumulation Variant Lines

研究了无胁迫和ＮａＣｌ胁迫条件下，同基因型二倍体葡萄４个脯氨酸累积变异系和对照系愈伤组织的过氧化氢酶（ＣＡＴ）和超氧物歧化酶（ＳＯＤ）活性。结果表明，在无胁迫条件下，葡萄脯氨酸累积变异系的ＣＡＴ和ＳＯＤ活性显著高于对照系，４个变异系的ＣＡＴ活性分别是对照的２．４１，２．３０，２．２９和２．１３倍，ＳＯＤ活性分别是对照的４．５１，３．６９，３．８４和３．７２倍。在一定浓度ＮａＣｌ胁迫下，对照系和变异系的ＣＡＴ和ＳＯＤ活性都有一定的增加，变异系的ＣＡＴ和ＳＯＤ活性仍高于对照系。

Activities of Catalase (CAT) and Superoxide dismutase (SOD) of four proline accumulation variant lines from homogeneous diploid grape somitic callus under NaClfree and NaClstress were studied.The result showed that CAT and SOD activities of grape proline accumulation variant lines under NaClfree condition were significantly higher than those of the control line.CAT activities of four variant lines HR5,HR1,HR2and HR6 were 2.41,2.30,2.29 and 2.32 times and SOD activities were 4.51, 3.69, 3.84 and 3.73 times as those of control line respectively.Under NaClstress conditions,CAT and SOD activities of four variant lines and control line were all increased,but variant lines still showed higher level of activities than their control line. It is suggested that high activities of CAT and SOD in the proline accumulation variant lines play an important role in variant lines more resistant to NaClstress than the control line.