HPLC测定血浆中尼美舒利浓度

Determination of nimesulide in plasma by HPLC

目的建立测定血浆中尼美舒利（Nim）的反相高效液相色谱法。方法血浆样品用乙腈沉淀蛋白，高速离心后取20出上清进样。以HigginsTMC18柱，流动相为有机相（甲醇：乙腈=400：275）：水相（乙酸：水=1：60）=70：30，缬沙坦为内标，在波长290Nm处检测，流速1．0mL／min，柱温：30℃。结果在0．11-11．0μg／mL浓度范围内，峰面积比与浓度呈良好线性关系，r=0．9996。最低检测限为0．11μg/mL。结论用本方法测定20例单剂量po100mgNim的血药浓度。结果满意。

Objective To determine the concentration of nimesulide in human plasma. Methods Blood plasma sample was treated by sedimenting proteinum with acetonitrile, and then high speed centrifugation was conducted to take supernatan20μl for HPLC injection. The drug and internal standard （valsartan） were chromatographed on HigginsTMC18 column with a mobile phase consisting of organic phase （ methanol： acetonitrile = 400：275 ） ： aqueous phase （ acetic acid： water = 1 ： 60 ） and detected at 290 nm. Chromatography was carried out at a flow rate of 1.0 ml/min at 30℃. Results A better linearity was obtained from 0.11μg to 11 μg of nimesulide per ml for plasma with a good correlation coefficient （ r = 0. 9996, n = 8）. The mininum detectable concentration was 0. 11 μg/ml for plasma. Conclusion Drug concentration in plasma was determined by HPLC method followed by a single oral dose of 100 mg given to 20 adult male volunteers. The procedure is presently being used in case work.