哮喘患者γ-谷氨酰半胱氨酸合成酶及还原型谷胱甘肽的活性变化

Changes of γ-glutamylcysteine synthetase and reduced glutathione in patients with bronchial asthma

目的探讨哮喘患者γ-谷氨酰半胱氨酸合成酶(γ-GCS)、还原型谷胱甘肽(GSH)的活性变化。方法选取哮喘急性发作期患者10例,按哮喘防治指南常规分级治疗6周,比较哮喘患者治疗前后诱导痰中γ-GCS、GSH、丙二醛(MDA)及血清中GSH、MDA和活性氧(ROS)的变化,同时进行肺功能检测和哮喘症状评分。选取10例健康志愿者作为正常对照。结果哮喘患者治疗前血清及诱导痰中GSH低于对照组(P<0.01)、MDA高于对照组(P<0.01),诱导痰中γ-GCS高于对照组(P<0.01)。治疗6周后,哮喘患者血清及诱导痰中GSH均较治疗前增高(P均<0.01),但仍低于对照组(P<0.05);血清及诱导痰中MDA、诱导痰中γ-GCS均较治疗前下降(P均<0.01),但仍高于对照组(P<0.05)。哮喘患者血清中GSH水平与哮喘症状评分、MDA、ROS水平呈负相关(r值分别为-0.701、-0.901和-0.878,P值分别<0.05、<0.01和<0.01),与FEV1%pred呈正相关(r=0.854,P<0.01)。哮喘患者诱导痰中γ-GCS活性与哮喘症状记分、MDA浓度呈正相关(r值分别为0.804和0.926,P值分别<0.05和<0.01)。结论γ-GCS、GSH参与哮喘患者的氧化/抗氧化反应。

Objective To explore changes of γ-glutamylcysteine synthetase（γ-GCS） and reduced glutathione（ GSH ） in patients with bronchial asthma. Methods Ten patients with acute asthma were enrolled and treated for six weeks according to guideline recommendations. Levels of γ-GCS, GSH and malondialdehyde（MDA） in total cells in induced sputum and GSH,MDA,reactive oxygen（ROS） in serum were measured and compared before and after therapy. Ten healthy volunteers were as normal control. Meanwhile ,the pulmonary function （ FEV1% pred ） was measured and asthmatic symptoms were quantified using Hogg＇s way. Results A. In serum and sputum of the asthma patients, GSH were lower and MDA were higher before treatment than those of the control （ P 〈 0. 01 ）. And γ-GCS in induced sputum were higher before treatment than those of control. B. After treated for six weeks, levels of GSH in serum and sputum of the asthma patients increased copmpared to baseline （ all P 〈 0. 01 ）, but were still lower than that of control （ P 〈 0. 05 ）. Activities of MDA in serum and sputum and γ-GCS in sputum were elevated compared to baseline（ P 〈 0.01 ）, but still higher than that of control（ all P 〈 0. 05 ）. C. Levels of GSH in serum of all patients were correlated negatively with asthmatic symptom scores and levels of MDA and ROS（r = -0. 701, -0. 901, -0. 878;P 〈0. 05, 〈0. 01, 〈0. 01）. There was a positive relationship between levels of GSH in serum and FEV1% pred （r = 0. 854, P 〈 0.01 ）. In induced sputum, activities of γ-GCS in all patients was correlated positively with their asthmatic symptom scores and level of MDA（ r = 0. 804,0. 926 ; P 〈 0. 05, 〈 0. 01 ） . Conclusion γ-GCS and GSH may participate the reaction of oxidation/antioxidation in patients with bronchial asthma.