淡紫拟青霉36-1菌株3种诱变育种方法的比较

STUDIES ON INDUCED MUTATIONAL METHODS TO Paecilomyces lilacinus 361

用孢子紫外照射、菌丝原生质体紫外照射和孢子６０Ｃｏγ射线辐射３种方法对淡紫拟青霉３６１菌株进行了诱变育种。结果表明，３种方法都能产生对Ｒｈｉｚｏｃｔｏｎｉａｓｏｌａｎｉ具高度拮抗活性的菌株，其中尤以孢子紫外线照射为佳。获得的２１２３、２２４５、４４１４等菌株的拮抗活性比３６１菌株提高了１００％以上。

Mutation was induced by means of UV and 60Coγ radiation to spores and UV to protoplast of hyphae to improve inhibiting ability of P.lilacinus 361 to Rhizoctonia solani. The results indicate that productive strains can be obtained from all these three methods. Among them of 1 min UV treatment to spores is the best one. After selecting, 2123,2245 and 4414 are more effective to R.solani than 361 by 100%.