福建省北部林区人群人粒细胞无形体血清流行病学检测

Serum epidemiological investigation on Anaplasma phagocytophila in population of the north forest area in Fujian province

目的了解福建省北部林区人群人粒细胞无形体病感染状况与分布特点,以及无形体与莱姆病、恙虫病重叠感染的情况,为林区人群无形体的预防提供科学依据。方法2007年7月开始在福建省武夷山林区采集林业工人、农民和干部等269份血清,用试剂盒采用间接免疫荧光法(IFA)检测人粒细胞无形体、莱姆病和恙虫病抗体,并进行不同地区、年龄、性别、职业分布比较。数据库建立和统计分析均采用SPSS14.0,χ2检验的显著性水平α=0.05。结果269份血清中检出46份人粒细胞无形体血清抗体阳性,阳性率17.10。人粒细胞无形体血清抗体除年龄外不存在地区、性别、职业差异。46份人粒细胞无形体抗体阳性的血清中检测到1份与莱姆病混合感染,9份混合感染了莱姆病和恙虫病病原体。结论福建省武夷山林区人群存在人粒细胞无形体感染,并且是莱姆病、恙虫病重叠流行区。在林区人粒细胞无形体病、莱姆病、恙虫病三者的防治应同时进行。

The aim of this study is to understand the present situation of Anaplasma phogocytophila infection and the coinfection with Borrelia burgdorferi and Orientia tsutsugarnushi in populations of the Wuyishan forest area of Fujian province, so as to provide scientific basis for the strategy of treatment and prevention.. Indirect fluorescence assay （IFA） was used to de- tect the presence of IgG antibodies against A. phagocytophila, B. burgdorferi and O. tsutsugarnushi in sera of workers, peasants and cadres at the basis in the forest area. All together, a total of 269 samples of serum were collected and tested. The establishment of database and statistical analysis were performed by SPSS 14.0 with a significance level of X^2 test at a= 0.05. It was found that among these 269 serum samples taken, 46 serum samples appeared to be positive for IgG antibody against A. phagocytophila, with a positive rate of 17. 10% and in these 46 positive samples for A. phagocytophila, one sample and 9 samples were positive for antibodies against B. burgdorferi and O. tsutsugamushi respectively. In the positive serum samples, no satistic difference existed in area, gender and occupation except age. It is concluded that endemic A. phagocytophila infection exists in the north forest area of Fujian province and co-infected with B. burgdorferi and O. tsutsugamushi. So the works of prevention and treatment of anaplasmosis, Lyme＇s disease and scrub typhus should be undertaken simultaneously.