JNK信号通路在δ氨基酮戊酸-光动力疗法诱导SW480细胞凋亡中的作用机制

Role of the c-Jun N-terminal kinase signaling pathway in SW480 cell apoptosis in response to 5-aminolevulinic acid-based photodynamic therapy

目的:探讨JNK信号通路在δ氨基酮戊酸(ALA)-光动力疗法((PDT)诱导SW480细胞凋亡中的作用.方法:将SW480细胞分为空白对照组、激光照射组、ALA组及ALA-PDT组,Western blot检测各组细胞c-Jun氨基末端激酶(JNK)的表达;用SP600125(JNK抑制剂)预孵育ALA-PDT组细胞,Western blot检测各组细胞的多聚(ADP-核糖)聚合酶(PARP)的表达.结果:磷酸化JNK在空白对照组、激光照射组及ALA组几乎无表达,ALA-PDT后30-90 min细胞表达显著高于空白对照组、激光照射组及ALA组(F=12.314,P<0.001),其中ALA-PDT后60及90 min的磷酸化JNK表达显著高于ALA-PDT后30 min(F=9.782,P<0.001),各组细胞总的JNK表达无显著差异.JNK的激活能抑制ALA-PDT诱导SW480细胞凋亡.结论:JNK信号通路的激活抑制ALA-PDT诱导SW480细胞凋亡;JNK通路可能成为增强ALA-PDT治疗结肠癌的新靶点.

AIM：To investigate the role of the c-Jun N-terminal kinase（JNK）signaling pathway in SW480 cell apoptosis in response to 5-aminolevulinic acid（ALA）-based photodynamic therapy（PDT）.METHODS：SW480 cells were divided into con-trol group,laser irradiation group,ALA group and ALA-PDT group.Western blot was used to detect expression of JNK in each group.SW480 cells in ALA-PDT group were preincubated with SP600125（JNK inhibitor）.Western blot was usedto detect expression of PARP in each group.RESULTS：JNK phosphorylation almost had no expression in control group,light group or ALA group.JNK phosphorylation of SW480 cells at 30,60 and 90 min after ALA-PDT was signifi-cantly increased than that of control group,light group or ALA group（F=12.314,P〈0.001）.Phosphorylated JNK expression was signifi-cantly higher at 60 min and 90 min after ALA-PDT than at 30 min after ALA-PDT（F=9.782,P〈0.001）.The expression of total JNK of all groups had no significant difference.The activa-tion of JNK inhibited apoptosis of SW480 cells in response to ALA-PDT.CONCLUSION：AActivation of JNK signaling pathway inhibits SW480 cell apoptosis after ALA-PDT.JNK signaling pathway may become a new target,which will enhance anticancer treatment of ALA-PDT for colon carcinoma.