铜绿假单胞菌随机扩增多态性DNA分子流行病学研究

Epidemiological Research on Chloropseudomonas Random Augmentation Polymorphism DNA Molecules.

目的:建立随机扩增多态性DNA(RAPD)基因分型方法监测重症监护室铜绿假单胞菌(PA)医院感染。方法:收集一年内从重症监护病房(ICU)分离出的21株铜绿假单胞菌,提取DNA后进行RAPD分型,同时进行抗生素敏感性实验。结果:21株PA共得14型,分型率100%;其中有3株属于同一种类型;抗生素敏感性实验表明21株均为对亚胺培南/西司他丁,头孢哌酮/舒巴坦等少数几种敏感的多重耐药株。结论:ICU内存在铜绿假单胞菌感染爆发流行,RAPD分型技术分型率高,简便快速,具有分子流行病学研究的应用价值。

Objective： To establish a method of randomized augmentation polymorphism DNA （RAPD） gene typing to monitor chloropseudomonas infection in ICU. Method： 21 chloropseudomonas strains collected and isolated from ICU during one year were typed in RAPS after refining DNA and were carried out the bacterial sensitivity test as well. Results： 14 types in 21 PA strains were obtained with typing rate 100% ; 3 of 21 strains belonged to the same type; the bacterial sensitivity test indicated that all the 21 strains were sensitive to a few multiple drug - resistant strain, such as imipenem/ cilastatin, cefoperazone/sulbactam, etc. Conclusion： There were Chloropseudomonas infection eruption in ICU. RAPD typing technique has a molecule epidemiology application value with high typing rate, simple and quick.