病理性瘢痕中VEGF与突变型P53基因表达的关系

Correlation of the expressions of VEGF and mutant P53 in pathogenesis scar and the clinical significance

目的研究病理性瘢痕中血管内皮生长因子(VEGF)与突变型抑癌基因P53的表达情况及其相互关系,探讨它们在病理性瘢痕形成中的作用及机制。方法应用免疫组化SP法检测正常皮肤、成熟瘢痕、增生性瘢痕和瘢痕疙瘩组织中VEGF和突变型P53基因的表达及其相关性。结果病理性瘢痕组织中VEGF的表达增高,与正常皮肤、成熟瘢痕对照组比较,差异有统计学意义(P<0.005)。增生性瘢痕与瘢痕疙瘩之间VEGF蛋白的表达,差异无统计学意义(P>0.05)。病理性瘢痕组织中突变型P53的表达增高,与正常皮肤、成熟瘢痕对照组比较差异有统计学意义(P<0.005)。增生性瘢痕与瘢痕疙瘩之间突变型P53蛋白的表达,差异无统计学意义(P>0.05)。VEGF与突变型P53蛋白表达,存在明显正相关(P<0.01)。结论VEGF与突变型P53在病理性瘢痕组织中均表达增高,与病理性瘢痕的形成密切相关,可能对病理性瘢痕的形成起着重要作用,两者之间存在正相关。

Objective To study the expression of vascular endothelial growth factor （VEGF） and mutant tumor suppressor gene P53 in the pathological scar and their probable role in the pathogenesis of abnormal scars. Methods Expression of VEGF and P53 in hypertrophic scar, keloid, mature scar and normal skin was examined by method. Results The expression of VEGF protein increased in abnormal scar（P 〈 0. 005） and their expression was not statistically defferent between the hypertrophic scar and keloid（P 〉0.05）. The level of mutant P53 protein expression was not satistically defferent between the hypertrophic scar and kelid（P 〉0.05）, while they were all remarkably significant in comparison between normal control group and abnormal scars（P 〈 0.005 ）. There was positive correlation existing between VEGF protein and mutant P53 protein expression （P 〈 0.01 ）. Conclusion The results indicated that the expression of VEGF and mutant P53 was increased in abnormal scar. VEGF and mutant P53 were tightly associated with the development of abnormal scar. The over- expression of VEGF and mutant P53 might play an important role in the pathogenes of pathological scar. The expression of VEGF positively related to the expression of mutant P53.