原发性肝癌患者血清中庚型肝炎病毒基因的检测

Detection of Hepatitis G Virus RNA in Serum from Patients with Primary Liver Cancer using PCR

用逆转录－聚合酶链反应法（ＰＴ－ＰＣＲ）检测了６７例原发性肝癌（ＰＬＣ）患者血清中的庚型肝炎病毒（ＨＧＶ）ＲＮＡ，以ＰＣＲ－双脱氧末端终止法分析了ＰＣＲ产物的核苷酸序列。结果显示，ＨＧＶＲＮＡ的检出率为１９．４％（１３／６７），其在ＨＢｓＡｇ阳性组和ＨＢｓＡｇ／抗ＨＣＶ阴性组中的阳性率分别是１２．２％（６／４９）和５０％（７／１４）；５’非编码区（５’－ＮＣＲ）扩增片段的核苷酸序列与美国株ＧＢＶ－Ｃ的同源性为９１．７％，提示在我国ＰＬＣ患者中存在ＨＧＶ感染。

Serum samples from 67 patients with primary liver cancer were detected for hepatitis G virus (HGV) RNA by reverse transcriptionpolymerase chain reaction (RTPCR) using primers from HGV 5 noncoding region 5 NCR. The nucleotide sequences of the PCR products were determined by dideoxynucleotide chain terminationPCR method. Thirteen out of 67 patients (19.4%) were positive for presence of HGV RNA. Seven out of 13 patients were positive for HBsAG and antiHCV.Compared with GBVC, the 5 NCR sequences of the PCR products showed 91.7% of homology. It is suggested that there is HGV infection in most of the patients with PLC in our country.