iKIR及其配体HLA—Cw表达与炎症性肠病遗传易感性的关系

Relationship between expression of inhibitory killer cell immunoglobulin-like receptor HLA-Cw ligand and susceptibility to inflammatory bowel disease

目的分析抑制性杀伤细胞免疫球蛋白样受体（iKIR）及其配体人类白细胞抗原（HLA）-Cw在炎症性肠病（IBD）患者中的表型分布，探讨其与IBD遗传易感性的关系。方法采用序列特异性引物聚合酶链反应（PCR—SSP）方法，分析100例溃疡性结肠炎（UC）、73例克罗恩病（CD）和106名种族匹配的健康对照（对照组）的iKIR基因表型和HLA—Cw分型、研究HLA-Cw与相应iKIR的识别情况。结果UC组中KIR2DLl和KIR2DL3表现型频率均明显低于对照组（0．710vs0．896、X^2=11．405、P=0．001，0．620vs0．821、X^2=10．362、P=0．001）；CD组中KIR2DL1表现型频率（0．740）也明显低于对照组（X^2=7．589、P=0．006）；UC患者和CD患者的KIR2DL1-HIJA—C2配对频率（2DL1^＋-HIJA—C2^＋分别为0．380和0．411）均低于对照组（0．575，X^2=7．876、P=0．005，X^2=4．681、P=0．030）。结论KIR2DL1-HLA—C2配对减少与IBD的易感性相关。

Objective To investigate the distribution of different phenotypes of inhibitory killer cell immunoglobutin-like receptor （iKIR） and its ligand HLA-Cw in patients with inflammatory bowel disease （ IBD）, and to explore whether iKIR/HLA-Cw combinations are associated with IBD susceptibility. Methods Peripheral blood samples were collected from 100 patients with ulcerative colitis （ UC）, 73 patients with Crohn＇s disease, and 106 randomly selected ethnically matched healthy controls. Polymerase chain reaction with sequence-specific primers was used to analyze the iKIR phenotypes, and the HLA-Cw phenotypes was examined with HLA-C LOCUS SSP UNITRAY kit, and the combination of HLA-Cw and its corresponding iKIR in individual was analyzed subsequently. Results The KIR2DL1 and KIR2DL3 gene phenotype frequencies in UC patients were 0. 710 and 0. 620 respectively, both significantly lower than those in healthy controls （0. 896, X^2 = 11. 405 P = 0. 001, and 0. 821, X^2 = 10.362 P = 0. 001 respectively）. The KIR2DL1 gene phenotype frequency in CD patients was 0. 740, significantly lower than that in healthy controls （0.896, X^2 = 7.589, P = 0.006）. The KIR2DL1 HLA-C2 combination （2DL1^＋-HLA-C2^＋ ） in UC patients and CD patients were 0. 380 and 0. 411 respectively, both significantly lower than that in healthy controls （0. 575, X^2 = 7. 876 P = 0. 005 and 0. 575, X^2 = 4. 681 P = 0. 030 respectively）. Conclusion The susceptibility to IBD is associated with decreased KIR2DL1-HLA-C2 combination.