转酮酶样基因TKTL1在人类鼻咽癌发生和转移中的作用

Effects of transketolase-like gene TKTL1 on occurrence and metastasis of human nasopharyngeal carcinoma

目的探讨转酮酶样基因TKTL1在人类鼻咽癌发生和转移中的作用。方法对65例鼻咽癌和9例鼻咽部慢性炎症组织中转酮酶活性进行检测；实时定量PCR检测鼻咽癌和鼻咽部慢性炎症组织中转酮酶基因家族（TKT,TKTL1和TKT12）mRNA表达水平的变化，并分析TKTL1的表达与鼻咽癌发生和转移的关系。结果鼻咽癌组织中转酮酶活性较鼻咽部慢性炎症组织明显增强（t=4．12，P=0．00）；实时定量PCR结果示鼻咽癌和鼻咽部慢性炎症组织中TKT和TKTL2 mRNA的表达水平差异无统计学意义（t=1．28、1．62，P=0．32、0．26）。然而，鼻咽癌组织中TKTL1的表达水平比鼻咽部慢性炎症组织明显上调（t=6．23，P=0．00），且有淋巴结转移的鼻咽癌组织中TKTL1的表达水平较无淋巴结转移者明显增强，差异有统计学意义（t=3．28，P=0．00）。结论转酮酶样基因TKTL1在人类鼻咽癌的发生和转移中可能起重要作用，TKTL1有望成为肿瘤治疗研究的新靶点。

Objective To investigate the effects of transketolase-like gene TKTL1 on the occurrence and metastasis of human nasopharyngeal carcinoma （NPC）. Methods Real-time PCR was used to determine the mRNA expression levels of transketolase gene family （TKT, TKTL1, and TKTL2） in the 65 biopsy specimens of human nasopharyngeal carcinoma, 9 at stage I ,15 at stage Ⅱ ,16 at stage Ⅲ ,13 at stage ⅣA, and 12 at stage ⅣB, 42 with metastasis and 232 without metastasis, and 9 biopsy specimens of chronic nasopharyngitiss. Results The TKL activity level of the NPC tissues was 21.6 ± 2. 8, significantly higher than that of the chronic nas@aryngitis tissues （ 6. 2 ± 1.3, P 〈0. 01 ）. The TKL activity level of the NPC tissues with metastasis was 26. 5 ± 3.2, significantly higher than that of the NPC tissues without metastasis （ 17. 6 ± 2. 1, P 〈 0.01 ）. There was no significant difference in the expression levels of TKT and TKTL2 genes between the human NPC and chronic nasopharyngitis tissues （both P 〉 0. 05）. The expression level of TKTL1 gene in the NPC tissues was 5.25 ± 0. 32, significantly higher than that in the chronic nasopharyngitis tissues （0. 98 ±0. 11, t =6. 23, P 〈0.01 ）, and the expression level of TKTL1 gene in the NPC tissues with lymph node metastasis was 5.86 ± 0. 38, significantly higher than that in the NPC tissues without lymph node metastasis （4. 18 ± 0. 22, t = 3.28, P 〈 0.01 ）. Conclusion TKTL1 may play an important role in the occurrence and metastasis of human NPC and become a potential target for novel anticancer therapy.