子宫颈病变中p16^(INK4A)的表达以及与HPV16感染关系的研究

Correlation of p16^(INK4A) expression with human papilloma virus 16 infection in cervical lesions

目的探讨p16INK4A在各类子宫颈病变中的表达以及与HPV16感染关系的研究,为临床子宫颈病变的筛查提供经济、有效、快速的筛查方法。方法应用组织微阵列方法和免疫组化法对15例正常子宫颈、10例子宫颈尖锐湿疣、45例子宫颈上皮内瘤样病变、20例子宫颈浸润癌行p16INK4A测定,用组织微阵列方法及原位杂交法行HPV16cDNA测定。结果随子宫颈病变的进展HPV16cDNA的表达增加(P<0.001)。p16INK4A在正常子宫颈中无表达,而随着子宫颈病变的进展,p16INK4A的表达率显著升高(P<0.001),且与HPV16有相关性。p16INK4A在子宫颈癌中的表达率为95.0。结论组织微阵列技术适用于大样本量的检测,是一种高效、快速、可比性强的分子生物学研究方法。p16INK4A在一定程度上可反映HPV的感染程度,可作为子宫颈癌筛查的指标。p16INK4A在子宫颈癌的发生中可能起着重要作用。

Objective To investigate the expression of pl6^INK4A in cervical lesions and its relation to human papilloma virus 16 （HPV16） infection. Methods p16^INK4A was detected by microarray and immunohistochemistry methods; HPV16cDNA was detected by microarray and in situ hybridization techniques in 15 tissue specimens of normal cervix, 10 cervical condyloma,45 CINs and 20 cervical carcinoma. Results The expression of HPV16cDNA increased with the progress of cervical lesions （P〈0. 001）. The expression of p16^INK4A was negative in all tissue specimens of normal cervix. The expression rate of p16^INK4A was significantly increased with the progress of cervical lesions （P〈0. 001）. The expression of p16^INK4A was positively correlated with that of HPV16cDNA. Conclusions The expression of p16^INK4A may reflect the severity of HPV infection. Detection of p16^NK4A with tissue microarray might be developed as a screening test for HPV infection.