非小细胞肺癌中p53基因突变的初步研究

Preliminary Study of p53 Gene Mutation in Human NonSmall Cell Lung Cancer

为了解非小细胞肺癌中ｐ５３基因突变的情况，应用改良聚合酶链反应－单链构象多态（ＰＣＲ－ＳＳＣＰ）银染技术检测１７例非小细胞肺癌中ｐ５３基因第５～８外显子的突变情况，有８例存在ｐ５３基因突变，突变率为４７．００％（８／１７），其中鳞癌为５５．５６％（５／９），腺癌为３７．５０％（３／８）。本研究结果表明：非小细胞肺癌中ｐ５３基因突变较为常见；

Using PCRSSCP silver staining technique, 17 cases of nonsmall cell lung cancer (NSCLC) were detected for mutations in p53 gene exon 5～8.Eight out of the 17 specimens tested showed p53 gene mutation positive yielding a total mutation rate of 47%. The mutation rates of squamous cell carcinoma and adenocarcinoma were 55.56% (5/9) and 37.50% (3/8) respectively. The results showed that p53 gene mutation is common in human NSCLC, and that PCRSSCP silver staining technique would be useful in gene diagnosis of NSCLC.