卵巢浆液性癌发生过程中ARHI STAT3和E2F1蛋白表达的变化及意义

Expression and clinical relevance of ARHI, STAT3 and E2F1 in ovarian serous carcinoma

目的研究卵巢浆液性癌由增生到癌变过程中，ARHI、STAT3和E2F1蛋白表达的变化规律及意义。方法采用免疫组化法，检测25例正常卵巢上皮组织、35例卵巢浆液性囊腺瘤、18例卵巢交界性浆液性囊腺瘤和56例卵巢浆液性囊腺癌的石蜡包埋组织中，ARHI、STAT3和E2F1蛋白的表达，分析3种蛋白表达与卵巢浆液性癌临床病理特征的关系。结果在25例正常卵巢上皮和35例浆液性囊腺瘤组织中，分别有22例和30例ARHI蛋白阳性表达，而在18例交界性囊腺瘤和56例浆液性囊腺癌组织中，只有10例和22例阳性表达，ARHI在浆液性癌和交界性囊腺瘤中的阳性表达率（39．3％和55．6％）明显低于其在正常卵巢和浆液性囊腺瘤中的表达（P〈0．05）。浆液性囊腺癌和交界性囊腺瘤组织中，STAT3蛋白的表达增强，其阳性表达率分别为87．5％（49／56）和77．8％（14／18），明显高于其在正常卵巢和卵巢浆液性囊腺瘤中的表达率（P〈0．05）。与正常卵巢、卵巢浆液性囊腺瘤和交界性囊腺瘤比较，卵巢浆液性癌中E2FI蛋白表达升高，阳性表达率为82．1％（46／56），明显高于前三者（P〈0．05）。统计分析显示，ARHI与STAT3和E2F1在卵巢浆液性癌中的蛋白表达呈显著的负相关关系。结论抑癌印迹基因ARHI的蛋白表达在卵巢浆液性癌中存在不同程度的降低或缺失，而STAT3和E2F1蛋白的表达明显增强，三者可能相互协同，在卵巢浆液性肿瘤的发生中发挥重要作用。

Objective To investigate the variation in expression of ARHI, STAT3 and E2F1 and the correlation among them during carcinogenesis of ovarian serous carcinoma. Methods Immunohistochemical staining was used to detect the expression of ARHI, STAT3 and E2F1 in samples of 25 normal ovaries, 35 ovarian serous cystadenomas, 18 borderline serous eystadenomas and 56 ovarian serous carcinomas. The variation in expression of the three genes and relationship among them were analyzed. Results ARHI expression was detected in 22 of 25 （ 88.0% ） normal ovaries and 30 of 35 （ 85.7% ） cystadenomas, but only in 10 of 18 （55.6%） borderline serous cystadenomas and 22 of 56 （39.3%） ovarian serous carcinomas, significantly lower than that in the normal ovaries and ovarian serous cystadenomas （ P 〈 0.05 ）. STAT3 expression was found in 14 of 18 （77.8%） borderline serous cystadenomas and 49 of 56 （87.5%） ovarian serous carcinomas, significantly higher than that in the normal ovaries and ovarian serous cystadenomas （P 〈 0.05 ）. To compare with E2F1 expression in the normal ovaries, serous eystadenomas and borderline serous cystadenomas, E2F1 expression in 46 of 56 （82.1% ） ovarian serous carcinomas was significantly higher （ P 〈 0.05 ）. It was found that the expression of ARHI was inversely correlated with that of STAT3 and E2F1. Conclusion Our findings indicate that ARHI expression is down-regulated, but STAT3 and E2F1 expressions are up-regulated, with an inverse correlation between ARHI and STAT3 in the carcinogenesis of ovarian serous carcinoma.