类风湿关节炎患者HLA-DR4/1亚型与抗环瓜氨酸肽抗体的关系

Relationship between HLA-DR4/1 and anti-cyclic citrullinated peptide antibody in rheumatoid arthritis

[目的]探讨类风湿关节炎(rheumatoid arthritis,RA)患者HLA-DR4/1分子与血清抗环瓜氨酸肽(cyclic cit-rullinated peptide,CCP)抗体之间的关系。[方法]收集118例RA患者,以序列特异性引物聚合酶链反应(PCR-SSP)技术对上述患者进行HLA-DR4/1分型,酶联免疫吸附试验(ELISA)方法检测患者血清抗CCP抗体水平。[结果]在118例RA患者中,35例患者HLA-DR4阳性,其中DRB1*0405阳性20例;5例患者HLA-DR1阳性。在118例RA患者中,抗CCP抗体阳性有90例。其中,在40例HLA-DR4/1阳性的RA患者中,抗CCP抗体阳性的占82.5%(33/40);在其余78例HLA-DR4/1阴性RA患者中,73.1%(57/78)抗CCP抗体阳性。HLA-DR4/1阳性患者与HLA-DR4/1阴性患者抗CCP抗体的阳性率差异无统计学意义(P=0.255)。而20例HLA-DRB1*0405阳性的RA患者抗CCP抗体阳性者19例,HLA-DRB1*0405阳性RA患者抗CCP抗体阳性率高于其他HLA-DR4/1亚型阳性的RA患者,也高于HLA-DR4/1阴性患者。[结论]RA中HLA-DRB1*0405基因与抗CCP抗体具有一定的相关性。

[ Objective] To explore the relationship between HLA- DR4/1 and anti -cyclic citrullinated peptide （CCP） antibody in rheumatoid arthritis （RA）. [ Methods] Serum were obtained from 118 RA patients. HLA -DR4/1 subtypes were analyzed by PCR- SSP. Antibodies to CCP were determined by ELISA. [ Results] Of 118 RA patients, 40 patients （33.9%） were positive for HLA - DR4/I subtypes including 20 HLA - DRB1^ ＊ 0405 positive patients, and 78 patients （66.1% ） were negative for HLA - DR4/I subtypes. In HLA - DR4/1 positive group, anti - CCP antibodies were present in 82.5 % RA （33/40）. In HLA- DR4/1 negative group, anti- CCP antibodies were present in 73.1% RA （57/78）. There was no significantly differences in production of antibodies to CCP between HLA - DR4/1 positive and HLA - DR4/ 1 negative groups. Whereas the positive rate of anti - CCP antibody in HLA - DRB1 ^＊ 0405 positive RA patients was higher than that of in other HLA - DR4/1 positive subtypes and HLA - DR4/1 negative RA patients. [ Conclusions ] There may be a relationship between HLA - DRB1^ ＊ 0405 subtypes and anti - CCP antibody.